Cellosaurus cell line CFBE41o- 6.2 WT-CFTR (CVCL

Cellosaurus CFBE41o- 6.2 WT-CFTR (CVCL_VP36)

[Text version]

Cell line name

CFBE41o- 6.2 WT-CFTR

Accession

CVCL_VP36

Resource Identification Initiative

To cite this cell line use: CFBE41o- 6.2 WT-CFTR (RRID:CVCL_VP36)

Comments

Genetic integration: Method=Transfection; Gene=HGNC; HGNC:1884; CFTR.
Genetic integration: Method=Transfection; Gene=UniProtKB; P00557; Escherichia coli hygromycin-B 4-O-kinase (hph) (HygR).
Transformant: NCBI_TaxID; 1891767; Simian virus 40 (SV40) (Note=pSVori-).
Caution: PubMed=18769032 describes two clones, CFBE41o- c7-6.2wt and CFBE41o- c10-6.2wt, but it is not possible to trace back which of the 2 clones is the one distributed as SCC160 (personal communication of Millipore).
Derived from site: In situ; Lung, bronchus, epithelium; UBERON=UBERON_0002031.
Cell type: Epithelial cell of bronchus; CL=CL_0002328.

Sequence variations

Mutation; HGNC; HGNC:1884; CFTR; Simple; p.Phe508del (c.1521_1523delCTT); ClinVar=VCV000007105; Zygosity=Unspecified; Note=In the 2 genomic alleles while the transfected copies are wild type (PubMed=18769032).

Disease

Cystic fibrosis (NCIt: C2975)
Cystic fibrosis (ORDO: Orphanet_586)

Species of origin

Homo sapiens (Human) (NCBI Taxonomy: 9606)

Hierarchy

Parent: CVCL_HL93 (CFBE41o-)

Sex of cell

Sex unspecified

Age at sampling

Age unspecified

Category

Transformed cell line

STR profile

Source(s): Millipore=SCC160

Markers:

Amelogenin	X
CSF1PO	11
D3S1358	16,18
D5S818	11,13
D7S820	8,11
D8S1179	11,14
D13S317	9,11
D16S539	12
D18S51	15,16
D21S11	31.2
FGA	22,24
Penta D	12
Penta E	12
TH01	9.3
TPOX	8,9
vWA	17

Publications

PubMed=18769032; DOI=10.1159/000149783; PMCID=PMC2927120
Beate Illek, Rosalie Maurisse, Logan Wahler, Karl Kunzelmann, Horst Fischer, Dieter C. Gruenert;
Cl transport in complemented CF bronchial epithelial cells correlates with CFTR mRNA expression levels.
Cell. Physiol. Biochem. 22:57-68(2008)

Cross-references

Cell line collections (Providers)

Millipore; SCC160

Encyclopedic resources

Wikidata; Q93447387

Entry history

Entry creation

07-Sep-2018

Last entry update

10-Apr-2025

Version number