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Cellosaurus HEK293-HM3 (CVCL_VP06)

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Cell line name HEK293-HM3
Synonyms HM3
Accession CVCL_VP06
Resource Identification Initiative To cite this cell line use: HEK293-HM3 (RRID:CVCL_VP06)
Comments Characteristics: Expresses the conditional kinase DeltaMEKK3:ER* which consist of the kinase domain of MAP3K3 fused in frame to a modified form of the hormone binding domain of the estrogen receptor (ESR1) that can be de-repressed by 4-hydroxytamoxifen (4-HT) but not beta-estradiol.
Genetic integration: Method=Transfection; Gene=HGNC; HGNC:3467; ESR1 (Note=Ligand-binding domain).
Genetic integration: Method=Transfection; Gene=HGNC; HGNC:6855; MAP3K3 (Note=Kinase domain).
Genetic integration: Method=Transfection; Gene=UniProtKB; P13249; Streptomyces alboniger pac (PuroR).
Transformant: NCBI_TaxID; 28285; Human adenovirus C serotype 5.
Derived from site: In situ; Fetal kidney; UBERON=UBERON_0002113.
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_0045 (HEK293)
Sex of cell Female
Age at sampling Fetus
Category Transformed cell line
Publications

PubMed=12444545; DOI=10.1038/sj.onc.1206000
Andrew P. Garner, Claire R. Weston, Daniel E. Todd, Kathryn Balmanno, Simon J. Cook;
Delta MEKK3:ER* activation induces a p38 alpha/beta 2-dependent cell cycle arrest at the G2 checkpoint.
Oncogene 21:8089-8104(2002)

PubMed=14981547; DOI=10.1038/sj.onc.1207467
Daniel E. Todd, Ruth M. Densham, Sarah A. Molton, Kathryn Balmanno, Catherine Newson, Claire R. Weston, Andrew P. Garner, Linda Scott, Simon J. Cook;
ERK1/2 and p38 cooperate to induce a p21CIP1-dependent G1 cell cycle arrest.
Oncogene 23:3284-3295(2004)

PubMed=16513653; DOI=10.1074/jbc.M510275200
Parjeet K. Boughan, Richard H. Argent, Mathilde Body-Malapel, Jong-Hwan Park, Katie E. Ewings, Andrew G. Bowie, Shao Jin Ong, Simon J. Cook, Ole E. Sorensen, Barbara A. Manzo, Naohiro Inohara ...Show all 15 authors... , Nigel J. Klein, Gabriel Nunez, John C. Atherton, Mona Bajaj-Elliott; Show fewer authors
Nucleotide-binding oligomerization domain-1 and epidermal growth factor receptor: critical regulators of beta-defensins during Helicobacter pylori infection.
J. Biol. Chem. 281:11637-11648(2006)

Cross-references
Cell line collections (Providers) CancerTools; 153328
Ximbio; 153328
Encyclopedic resources Wikidata; Q94095208
Entry history
Entry creation07-Sep-2018
Last entry update14-Aug-2025
Version number9