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Cellosaurus HeLa/Fucci(CA)2 (CVCL_VM98)

[Text version]
Cell line name HeLa/Fucci(CA)2
Synonyms HeLa/Fucci(CA)2 #3
Accession CVCL_VM98
Resource Identification Initiative To cite this cell line use: HeLa/Fucci(CA)2 (RRID:CVCL_VM98)
Comments Population: African American.
Characteristics: Used for study of cell cycle progression thanks to the inclusion of a Fucci(CA2) (Fluorescent Ubiquitination-based Cell Cycle Indicator) construct.
Characteristics: Transfected with the Fucci(CA)2 probe which consist of the fusion of mCherry to the ubiquitination domain of human CDT1 (aa 1-100) but with the Cy motif 68-RRL-70 replaced by AAA and of monomeric Venus (mVenus) to the ubiquitination domain of human geminin (aa 1-110).
Genetic integration: Method=Transfection; Gene=FPbase; ZERB6; mCherry (Note=Red fluorescent protein derived from drFP583 (dsRed)).
Genetic integration: Method=Transfection; Gene=FPbase; YUJWJ; Venus (Note=Bright yellow fluorescent protein derived from GFP).
Genetic integration: Method=Transfection; Gene=HGNC; HGNC:24576; CDT1 (Note=With p.Ser101_Leu546del and p.Arg68Ala, p.Arg69Ala and p.Leu70Ala).
Genetic integration: Method=Transfection; Gene=HGNC; HGNC:17493; GMNN (Note=With p.Tyr111_Ile209del).
Transformant: NCBI_TaxID; 333761; Human papillomavirus type 18 (HPV18).
Derived from site: In situ; Uterus, cervix; UBERON=UBERON_0000002.
Disease Human papillomavirus-related endocervical adenocarcinoma (NCIt: C27677)
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_0058 (HeLa S3)
Sex of cell Female
Age at sampling 30Y6M
Category Cancer cell line
Publications

PubMed=29107535; DOI=10.1016/j.molcel.2017.10.001
Asako Sakaue-Sawano, Masahiro Yo, Naoki Komatsu, Toru Hiratsuka, Takako Kogure, Tetsushi Hoshida, Naoki Goshima, Michiyuki Matsuda, Hiroyuki Miyoshi, Atsushi Miyawaki;
Genetically encoded tools for optical dissection of the mammalian cell cycle.
Mol. Cell 68:626-640.e5(2017)

Cross-references
Cell line collections (Providers) RCB; RCB4676
Encyclopedic resources Wikidata; Q94101207
Entry history
Entry creation07-Sep-2018
Last entry update10-Apr-2025
Version number12