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Cellosaurus CRISPRi Gen3C A4 (CVCL_VM43)

[Text version]

Cell line name CRISPRi Gen3C A4
Synonyms WTC CRISPRi Gen3 clone A4; 3C-A4
Accession CVCL_VM43
Resource Identification Initiative To cite this cell line use: CRISPRi Gen3C A4 (RRID:CVCL_VM43)
Comments Population: Japanese.
Characteristics: Cell line with constitutive CRISPR interference (CRISPRi).
Characteristics: Using TALEN a construct containing a CAG promoter and dCas9-KRAB is introduced in the AAVS1 locus.
Transfected with: UniProtKB; Q99ZW2; Streptomyces pyogenes Cas9.
Derived from sampling site: Leg; skin. Cell type=Fibroblast.
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_Y803 (GM25256)
Sex of cell Male
Age at sampling 30Y
Category Induced pluripotent stem cell

PubMed=26971820; DOI=10.1016/j.stem.2016.01.022
Mandegar M.A., Huebsch N., Frolov E.B., Shin E., Truong A., Olvera M.P., Chan A.H., Miyaoka Y., Holmes K., Spencer C.I., Judge L.M., Gordon D.E., Eskildsen T.V., Villalta J.E., Horlbeck M.A., Gilbert L.A., Krogan N.J., Sheikh S.P., Weissman J.S., Qi L.S., So P.-L., Conklin B.R.
CRISPR interference efficiently induces specific and reversible gene silencing in human iPSCs.
Cell Stem Cell 18:541-553(2016)

Encyclopedic resources Wikidata; Q93458774
Entry history
Entry creation07-Sep-2018
Last entry update17-Mar-2022
Version number4