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Cellosaurus CRISPRi Gen1C 6 (CVCL_VM39)

[Text version]

Cell line name CRISPRi Gen1C 6
Synonyms WTC CRISPRi Gen1 clone 6; 1C-6
Accession CVCL_VM39
Resource Identification Initiative To cite this cell line use: CRISPRi Gen1C 6 (RRID:CVCL_VM39)
Comments Population: Japanese.
Characteristics: Cell line with Dox-inducible CRISPR interference (CRISPRi).
Characteristics: Using TALEN a construct containing a rtTA+TRE3G promoter and dCas9-KRAB-P2A-mCherry is introduced in the AAVS1 locus.
Transfected with: UniProtKB; Q99ZW2; Streptomyces pyogenes Cas9.
Transfected with: UniProtKB; X5DSL3; Anaplasma marginale mCherry fluorescent protein.
Derived from sampling site: Leg; skin. Cell type=Fibroblast.
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_Y803 (GM25256)
Sex of cell Male
Age at sampling 30Y
Category Induced pluripotent stem cell
Publications

PubMed=26971820; DOI=10.1016/j.stem.2016.01.022
Mandegar M.A., Huebsch N., Frolov E.B., Shin E., Truong A., Olvera M.P., Chan A.H., Miyaoka Y., Holmes K., Spencer C.I., Judge L.M., Gordon D.E., Eskildsen T.V., Villalta J.E., Horlbeck M.A., Gilbert L.A., Krogan N.J., Sheikh S.P., Weissman J.S., Qi L.S., So P.-L., Conklin B.R.
CRISPR interference efficiently induces specific and reversible gene silencing in human iPSCs.
Cell Stem Cell 18:541-553(2016)

Cross-references
Encyclopedic resources Wikidata; Q93458765
Entry history
Entry creation07-Sep-2018
Last entry update17-Mar-2022
Version number5