Cellosaurus cell line A-375 KRAS p.G13D-Luc2 (CVCL

Cellosaurus A-375 KRAS p.G13D-Luc2 (CVCL_UR34)

[Text version]

Cell line name

A-375 KRAS p.G13D-Luc2

Synonyms

KRAS mutant-A375 Isogenic-Luc2

Accession

CVCL_UR34

Resource Identification Initiative

To cite this cell line use: A-375 KRAS p.G13D-Luc2 (RRID:CVCL_UR34)

Comments

Population: Caucasian.
Characteristics: Exhibits significant resistance to the BRAF inhibitors Dabrafineb and Vemurafenib when compared to its parental cell line. Can be a useful model to study the RAS-RAF-MEK-ERK-MAP kinase signaling pathway and to screen potential BRAF inhibitors and anti-cancer compounds for drug discovery and development (ATCC=CRL-1619IG-1-LUC2).
Characteristics: Stably expresses firefly luciferase under the control of the human EF1a promoter.
Transfected with: UniProtKB; P08659; Firefly luciferase (with optimized codon usage for mammalian expression).
Transfected with: UniProtKB; P33967; B.cereus Bsr.
Derived from site: In situ; Skin; UBERON=UBERON_0002097.

Sequence variations

Mutation; HGNC; 1097; BRAF; Simple; p.Val600Glu (c.1799T>A); ClinVar=VCV000013961; Zygosity=Homozygous (from parent cell line).
Mutation; HGNC; 1787; CDKN2A; Simple; p.Glu61Ter (c.181G>T) (p.Gly75Val, c.224G>T); ClinVar=VCV000376309; Zygosity=Homozygous (from parent cell line).
Mutation; HGNC; 1787; CDKN2A; Simple; p.Glu69Ter (c.205G>T) (p.Gly83Val, c.248G>T); ClinVar=VCV000376308; Zygosity=Homozygous (from parent cell line).
Mutation; HGNC; 6407; KRAS; Simple_edited; p.Gly13Asp (c.38G>A); ClinVar=VCV000012580; Zygosity=Heterozygous; Note=By CRISPR/Cas9 (ATCC=CRL-1619IG-1-LUC2).
Mutation; HGNC; 11730; TERT; Simple; c.1-146C>T (c.250C>T) (C250T); Zygosity=Unspecified; Note=In promoter (from parent cell line).

Disease

Amelanotic melanoma (NCIt: C3802)

Species of origin

Homo sapiens (Human) (NCBI Taxonomy: 9606)

Hierarchy

Parent: CVCL_UD29 (A-375 KRAS p.G13D)

Sex of cell

Female

Age at sampling

54Y