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Cellosaurus HeLa GFP-LC3-RFP-LC3deltaG (CVCL_UP51)

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Cell line name HeLa GFP-LC3-RFP-LC3deltaG
Synonyms GFP-LC3-RFP-LC3deltaG HeLa
Accession CVCL_UP51
Resource Identification Initiative To cite this cell line use: HeLa GFP-LC3-RFP-LC3deltaG (RRID:CVCL_UP51)
Comments Population: African American.
Characteristics: Stably expresses EGFP-LC3-mRFP-LC3delta, an autophagic flux probe. GFP-LC3 is fused to the N-terminus of RFP-LC3 whose C-terminal glycine is deleted. When expressed, the probe it is separated into EGFP-LC3 and mRFP-LC3deltaG by the action of endogenous ATG4 family proteases that cleave the peptide bond after the C-terminal glycine of LC3 in the cytosol producing equimolar amounts of EGFP-LC3 and mRFP-LC3deltaG.
Transfected with: RGD; 735183; Map1lc3a.
Transfected with: UniProtKB; P42212; GFP (with p.Phe64Leu, p.Ser65Thr and p.His231Leu = EGFP).
Transfected with: UniProtKB; A0A4V4ND72; mRFP1.
Transformant: NCBI_TaxID; 333761; Human papillomavirus type 18 (HPV18).
Derived from site: In situ; Uterus, cervix; UBERON=UBERON_0000002.
Disease Human papillomavirus-related endocervical adenocarcinoma (NCIt: C27677)
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_0030 (HeLa)
Sex of cell Female
Age at sampling 30Y6M
Category Cancer cell line
Publications

PubMed=27818143; DOI=10.1016/j.molcel.2016.09.037
Kaizuka T., Morishita H., Hama Y., Tsukamoto S., Matsui T., Toyota Y., Kodama A., Ishihara T., Mizushima T., Mizushima N.
An autophagic flux probe that releases an internal control.
Mol. Cell 64:835-849(2016)

Cross-references
Cell line collections (Providers) RCB; RCB4695
Encyclopedic resources Wikidata; Q94096465
Entry history
Entry creation25-Feb-2019
Last entry update29-Jun-2023
Version number6