Cellosaurus HEK-Blue TNF-alpha (CVCL_UF25)
| Cell line name | HEK-Blue TNF-alpha |
|---|---|
| Accession | CVCL_UF25 |
| Resource Identification Initiative | To cite this cell line use: HEK-Blue TNF-alpha (RRID:CVCL_UF25) |
| Comments | Characteristics: Allows the detection of bioactive human and murine TNF-alpha by monitoring the activation of the NF-kappaB pathway (InvivoGen=hkb-tnfdmyd). Characteristics: SEAP (secreted ALPP) reporter cell line, in this cell line SEAP is under the control of an IFN-beta minimal promoter fused to 5 NF-kappaB and AP-1-binding sites (InvivoGen=hkb-tnfdmyd). Knockout cell: Method=Not specified; HGNC; HGNC:7562; MYD88. Genetic integration: Method=Transfection; Gene=HGNC; HGNC:11916; TNFRSF1A. Genetic integration: Method=Transfection; Gene=HGNC; HGNC:11917; TNFRSF1B. Genetic integration: Method=Transfection; Gene=HGNC; HGNC:439; ALPP. Genetic integration: Method=Transfection; Gene=UniProtKB; P13249; S.alboniger pac (PuroR). Genetic integration: Method=Transfection; Gene=UniProtKB; P17493; Streptoalloteichus hindustanus ble (BleoR). Transformant: NCBI_TaxID; 28285; Adenovirus 5. Derived from site: In situ; Fetal kidney; UBERON=UBERON_0002113. |
| Species of origin | Homo sapiens (Human) (NCBI Taxonomy: 9606) |
| Hierarchy | Parent: CVCL_0045 (HEK293) |
| Sex of cell | Female |
| Age at sampling | Fetus |
| Category | Transformed cell line |
| Cross-references | |
| Cell line collections (Providers) | InvivoGen; hkb-tnfdmyd |
| Encyclopedic resources | Wikidata; Q94092462 |
| Entry history | |
| Entry creation | 13-Nov-2018 |
| Last entry update | 10-Apr-2025 |
| Version number | 9 |