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Cellosaurus NP-2/CD4/CCR5/iGFP (CVCL_UF02)

[Text version]
Cell line name NP-2/CD4/CCR5/iGFP
Accession CVCL_UF02
Resource Identification Initiative To cite this cell line use: NP-2/CD4/CCR5/iGFP (RRID:CVCL_UF02)
Comments Population: Japanese.
Characteristics: GFP-inducible indicator cell line produced by transfection of the pHIV-LTR-GFP-NLS plasmid, containing HIV-1 long terminal repeat (LTR), eGFP, the nuclear localization signal (NLS) of HIV-1 Rev and pac.
Genetic integration: Method=Transfection/transduction; Gene=FPbase; R9NL8; eGFP (Note=Enhanced GFP).
Genetic integration: Method=Transfection/transduction; Gene=HGNC; HGNC:1606; CCR5.
Genetic integration: Method=Transfection/transduction; Gene=HGNC; HGNC:1678; CD4.
Genetic integration: Method=Transfection/transduction; Gene=UniProtKB; P00552; Klebsiella pneumoniae transposon Tn5 neo.
Genetic integration: Method=Transfection/transduction; Gene=UniProtKB; P13249; Streptomyces alboniger pac (PuroR).
Derived from site: In situ; Brain, left frontal lobe; UBERON=UBERON_0002811.
Sequence variations
  • Mutation; HGNC; HGNC:11998; TP53; Simple; p.Met237Ile (c.711G>T); ClinVar=VCV000634770; Zygosity=Unspecified (from parent cell line).
Disease Glioblastoma (NCIt: C3058)
Glioblastoma (ORDO: Orphanet_360)
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_UF00 (NP-2/CD4/iGFP)
Sex of cell Male
Age at sampling 63Y
Category Cancer cell line
Publications

DOI=10.2974/kmj.62.1
Hiroo Hoshino;
The NP-2 cell system for analyses of the coreceptor usage of human and simian immunodeficiency viruses.
Kitakanto Med. J. 62:1-14(2012)

PubMed=22178016; DOI=10.1016/j.micinf.2011.11.005
Sheikh Ariful Hoque, Takahiro Ohtsuki, Masashi Tatsumi, Nobuaki Shimizu, Salequl Islam, Atsushi Jinno-Oue, Hiroo Hoshino;
Lack of the trans-receptor mechanism of HIV-1 infection: CD4- and coreceptor-independent incorporation of HIV-1-resistant cells into syncytia induced by HIV-1.
Microbes Infect. 14:357-368(2012)

Cross-references
Cell line databases/resources cancercelllines; CVCL_UF02
Encyclopedic resources Wikidata; Q98128010
Entry history
Entry creation13-Nov-2018
Last entry update14-Aug-2025
Version number11