Cellosaurus cell line hCMEC/D3 (CVCL

Cellosaurus hCMEC/D3 (CVCL_U985)

[Text version]

Cell line name

hCMEC/D3

Synonyms

HCMEC/D3; CMEC/D3; human Cortical Microvessels Endothelial Cells/D3

Accession

CVCL_U985

Resource Identification Initiative

To cite this cell line use: hCMEC/D3 (RRID:CVCL_U985)

Comments

Group: Patented cell line.
Registration: International Depositary Authority, Pasteur Institute Collection Nationale de Cultures de Micro-organismes (CNCM); I-3308.
Genetic integration: Method=Transduction; Gene=HGNC; HGNC:11730; TERT.
Genetic integration: Method=Transduction; Gene=UniProtKB; P03070; Simian virus 40 (SV40) large T antigen.
Transformant: NCBI_TaxID; 1891767; Simian virus 40 (SV40).
Omics: Proteomics; Subcellular; Membrane; Quantitative.
Omics: Proteomics; Subcellular; Secretome.
Derived from site: In situ; Brain, temporal lobe, blood microvessel; UBERON=UBERON_0001871+UBERON_8410081.
Cell type: Endothelial cell; CL=CL_0000115.

Species of origin

Homo sapiens (Human) (NCBI Taxonomy: 9606)

Hierarchy

Children:

CVCL_C8XS (HCMEC/D3-Luc)	CVCL_E2Q2 (HyCyte hCMEC/D3 hRNF213_p.R4810K_c.12429G>A)	CVCL_E1K0 (HyCyte hCMEC/D3 KO-hRNF213)
CVCL_D8GX (Ubigene hCMEC/D3 JAM2 KO)	CVCL_D8GY (Ubigene hCMEC/D3 JAM3 KO)

Sex of cell

Female

Age at sampling

Adult

Category

Transformed cell line

STR profile

Source(s): CCRID=5301HUM-KCB19022YJ; Millipore=SCC066; Technion Genomics Center

Markers:

Amelogenin	X
CSF1PO	10,12
D1S1656	15.3,17.3
D2S441	13
D2S1338	16,20
D3S1358	16,17
D5S818	11
D7S820	10,12
D8S1179	15,16
D10S1248	14,15
D12S391	19.3,22
D13S317	11,14
D16S539	9,12
D18S51	12,19
D21S11	30,32.2
D22S1045	11,15
FGA	22,26
Penta D	10,12
Penta E	5,10
TH01	7,9
TPOX	10,11
vWA	14,17

Web pages

Provider; Cedarlane; CLU512; https://www.cedarlanelabs.com/Products/Detail/CLU512?lob=AllProducts
Provider; Cornell TTO; -; https://cornell.flintbox.com/technologies/bc9b2b68-4f6b-4071-bece-64241c21e438

Publications

PubMed=16141364; DOI=10.1096/fj.04-3458fje
Babette B. Weksler, Eva-Anne Subileau, Nicolas Perriere, Pierre Charneau, Karen A. Holloway, Mathilde Leveque, Helene Tricoire-Leignel, Ambra Nicotra, Sandrine Bourdoulous, Pawel Turowski, David K. Male ...Show all 15 authors... , Francoise Roux, John Greenwood, Ignacio A. Romero, Pierre-Olivier Couraud; Show fewer authors
Blood-brain barrier-specific properties of a human adult brain endothelial cell line.
FASEB J. 19:1872-1874(2005)

Patent=US8084254
Pierre-Olivier Couraud, Ignacio A. Romero, Babette B. Weksler;
Human blood brain barrier model.
Patent number US8084254, 27-Dec-2011

PubMed=23531482; DOI=10.1186/2045-8118-10-16; PMCID=PMC3623852
Babette B. Weksler, Ignacio A. Romero, Pierre-Olivier Couraud;
The hCMEC/D3 cell line as a model of the human blood brain barrier.
Fluids Barriers CNS 10:16.1-16.10(2013)

PubMed=26718731; DOI=10.1016/j.jprot.2015.12.011
Victor Llombart, Teresa Garcia-Berrocoso, Joan Josep Bech-Serra, Alba Simats, Alejandro Bustamante, Dolors Giralt, Gemma Reverter-Branchat, Francesc Canals, Mar Hernandez-Guillamon, Joan Montaner;
Characterization of secretomes from a human blood brain barrier endothelial cells in-vitro model after ischemia by stable isotope labeling with aminoacids in cell culture (SILAC).
J. Proteomics 133:100-112(2016)

PubMed=30983357; DOI=10.1021/acs.molpharmaceut.9b00114
Takeshi Masuda, Toshiki Hoshiyama, Tatsuki Uemura, Mio Hirayama-Kurogi, Seiryo Ogata, Arisu Furukawa, Pierre-Olivier Couraud, Tomomi Furihata, Shingo Ito, Sumio Ohtsuki;
Large-scale quantitative comparison of plasma transmembrane proteins between two human blood-brain barrier model cell lines, hCMEC/D3 and HBMEC/cibeta.
Mol. Pharm. 16:2162-2171(2019)

Cross-references

Cell line collections (Providers)

BCRJ; 0401
CLS; 305024
KCB; KCB 2019022YJ
Millipore; SCC066
Ubigene; YC-C029

Cell line databases/resources

CCRID; 5301HUM-KCB19022YJ

Anatomy/cell type resources

BTO; BTO_0003339

Chemistry resources

ChEMBL-Cells; CHEMBL4513105
PubChem_Cell_line; CVCL_U985

Encyclopedic resources

Wikidata; Q54881892

Proteomic databases