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Cellosaurus HeLa/Fucci2 (CVCL_U187)

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Cell line name HeLa/Fucci2
Synonyms HeLa/Fucci2 #3; HeLa.S-Fucci2; HeLa/Fucci(SA)2
Accession CVCL_U187
Resource Identification Initiative To cite this cell line use: HeLa/Fucci2 (RRID:CVCL_U187)
Comments Population: African American.
Characteristics: Used for study of cell cycle progression thanks to the inclusion of a Fucci2 (Fluorescent Ubiquitination-based Cell Cycle Indicator 2) construct.
Characteristics: Transfected with the Fucci2 probe which consist of the fusion of mCherry) to the ubiquitination domain of human CDT1 (AA 30-120) and of monomeric Venus (mVenus) to the ubiquitination domain of human geminin (AA 1-110).
Genetic integration: Method=Transfection; Gene=FPbase; ZERB6; mCherry (Note=Red fluorescent protein derived from drFP583 (dsRed)).
Genetic integration: Method=Transfection; Gene=FPbase; YUJWJ; Venus (Note=Bright yellow fluorescent protein derived from GFP).
Genetic integration: Method=Transfection; Gene=HGNC; HGNC:24576; CDT1 (Note=With p.Met1_Thr29del and p.Ser121_Leu546del).
Genetic integration: Method=Transfection; Gene=HGNC; HGNC:17493; GMNN (Note=With p.Tyr111_Ile209del).
Transformant: NCBI_TaxID; 333761; Human papillomavirus type 18 (HPV18).
Derived from site: In situ; Uterus, cervix; UBERON=UBERON_0000002.
Disease Human papillomavirus-related endocervical adenocarcinoma (NCIt: C27677)
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_0030 (HeLa)
Sex of cell Female
Age at sampling 30Y6M
Category Cancer cell line
Publications

PubMed=21226962; DOI=10.1186/1471-2121-12-2; PMCID=PMC3277280
Asako Sakaue-Sawano, Tamiyo Kobayashi, Kenji Ohtawa, Atsushi Miyawaki;
Drug-induced cell cycle modulation leading to cell-cycle arrest, nuclear mis-segregation, or endoreplication.
BMC Cell Biol. 12:2.1-2.12(2011)

PubMed=29107535; DOI=10.1016/j.molcel.2017.10.001
Asako Sakaue-Sawano, Masahiro Yo, Naoki Komatsu, Toru Hiratsuka, Takako Kogure, Tetsushi Hoshida, Naoki Goshima, Michiyuki Matsuda, Hiroyuki Miyoshi, Atsushi Miyawaki;
Genetically encoded tools for optical dissection of the mammalian cell cycle.
Mol. Cell 68:626-640.e5(2017)

Cross-references
Cell line collections (Providers) RCB; RCB2867
Cell line databases/resources CLO; CLO_0050932
Chemistry resources ChEMBL-Cells; CHEMBL4630774
ChEMBL-Targets; CHEMBL4630782
PubChem_Cell_line; CVCL_U187
Encyclopedic resources Wikidata; Q54882692
Entry history
Entry creation03-Feb-2014
Last entry update10-Apr-2025
Version number17