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Cellosaurus HeLa/Fucci2 (CVCL_U187)

[Text version]
Cell line name HeLa/Fucci2
Synonyms HeLa/Fucci2 #3; HeLa.S-Fucci2; HeLa/Fucci(SA)2
Accession CVCL_U187
Resource Identification Initiative To cite this cell line use: HeLa/Fucci2 (RRID:CVCL_U187)
Comments Population: African American.
Characteristics: Used for study of cell cycle progression thanks to the inclusion of a Fucci2 (Fluorescent Ubiquitination-based Cell Cycle Indicator 2) construct.
Characteristics: Transfected with the Fucci2 probe which consist of the fusion of mCherry) to the ubiquitination domain of human CDT1 (aa 30-120) and of monomeric Venus (mVenus) to the ubiquitination domain of human geminin (aa 1-110).
Transfected with: HGNC; 24576; CDT1 (with p.Met1_Thr29del and p.Ser121_Leu546del).
Transfected with: HGNC; 17493; GMNN (with p.Tyr111_Ile209del).
Transfected with: UniProtKB; X5DSL3; Anaplasma marginale mCherry fluorescent protein.
Transfected with: Venus, a bright yellow fluorescent protein derived from YFP.
Transformant: NCBI_TaxID; 333761; Human papillomavirus type 18 (HPV18).
Derived from site: In situ; Uterus, cervix; UBERON=UBERON_0000002.
Disease Human papillomavirus-related cervical adenocarcinoma (NCIt: C27677)
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_0030 (HeLa)
Sex of cell Female
Age at sampling 30Y6M
Category Cancer cell line

PubMed=21226962; DOI=10.1186/1471-2121-12-2
Sakaue-Sawano A., Kobayashi T., Ohtawa K., Miyawaki A.
Drug-induced cell cycle modulation leading to cell-cycle arrest, nuclear mis-segregation, or endoreplication.
BMC Cell Biol. 12:2.1-2.12(2011)

PubMed=29107535; DOI=10.1016/j.molcel.2017.10.001
Sakaue-Sawano A., Yo M., Komatsu N., Hiratsuka T., Kogure T., Hoshida T., Goshima N., Matsuda M., Miyoshi H., Miyawaki A.
Genetically encoded tools for optical dissection of the mammalian cell cycle.
Mol. Cell 68:626-640(2017)

Cell line collections (Providers) RCB; RCB2867
Cell line databases/resources CLO; CLO_0050932
Chemistry resources ChEMBL-Cells; CHEMBL4630774
ChEMBL-Targets; CHEMBL4630782
PubChem_Cell_line; CVCL_U187
Encyclopedic resources Wikidata; Q54882692
Entry history
Entry creation03-Feb-2014
Last entry update05-Oct-2023
Version number14