Cellosaurus cell line K-562 CRISPRa (CVCL

Cellosaurus K-562 CRISPRa (CVCL_RY26)

[Text version]

Cell line name

K-562 CRISPRa

Synonyms

K562 dCas9-SunTag-VP64

Accession

CVCL_RY26

Resource Identification Initiative

To cite this cell line use: K-562 CRISPRa (RRID:CVCL_RY26)

Comments

Population: Caucasian.
Characteristics: This cell line is transfected by a nuclease-deficient mutant of SpCas9 (dCas9) under the control of a promoter containing 4 copies of the activating domain of HSV-1 VP16 (termed VP64) and to 24 copies of a short peptide epitope (from yeast GCN4) which is termed SunTag.
Genetic integration: Method=Transduction; Gene=UniProtKB; Q99ZW2; Streptomyces pyogenes Cas9 (Note=With p.Asp10Ala and p.His840Ala = dCas9).
Derived from site: In situ; Pleural effusion; UBERON=UBERON_0000175.

Sequence variations

Gene fusion; HGNC; HGNC:76; ABL1 + HGNC; HGNC:1014; BCR; Name(s)=BCR-ABL1, BCR-ABL; Note=BCR exon 14 fused to ABL1 exon 2 (b3a2 transcript) (from parent cell line).
Mutation; HGNC; HGNC:11998; TP53; Simple; p.Gln136fs*13 (c.406_407insC); Zygosity=Homozygous (from parent cell line).

Disease

Blast phase chronic myelogenous leukemia, BCR-ABL1 positive (NCIt: C9110)
Chronic myeloid leukemia (ORDO: Orphanet_521)

Species of origin

Homo sapiens (Human) (NCBI Taxonomy: 9606)

Hierarchy

Parent: CVCL_0004 (K-562)
Children:

CVCL_RY28 (K-562 CRISPRa+SaCas9 clone C11)

CVCL_RY27 (K-562 CRISPRa+SaCas9 clone C7)

Sex of cell

Female

Age at sampling

53Y