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Cellosaurus BSR-T7/5 (CVCL_RW96)

[Text version]
Cell line name BSR-T7/5
Synonyms BsR-T7/5; BSR T7/5; BHK-T7
Accession CVCL_RW96
Resource Identification Initiative To cite this cell line use: BSR-T7/5 (RRID:CVCL_RW96)
Comments Characteristics: Expression of the T7 RNA polymerase gene is under control of the cytomegalovirus promoter (PubMed=9847328).
Genetic integration: Method=Transfection; Gene=UniProtKB; P00573; Bacteriophage T7 DNA-directed RNA polymerase.
Genetic integration: Method=Transfection; Gene=UniProtKB; P00552; Klebsiella pneumoniae transposon Tn5 neo.
Caution: This cell line is protected by IP rights of the inventors. The CCLV of the Friedrich-Loeffler-Institute, Federal Research Center for Virus Diseases of Animals is the sole approved distributor is CCLV (personal communication of Finke, Stefan).
Derived from site: In situ; Kidney; UBERON=UBERON_0002113.
Species of origin Mesocricetus auratus (Golden hamster) (NCBI Taxonomy: 10036)
Hierarchy Parent: CVCL_1915 (BHK-21 clone 13)
Children:
CVCL_E3AZ (BSR-T7/5 CL21)
Sex of cell Male
Age at sampling 1D
Category Spontaneously immortalized cell line
Publications

PubMed=9847328; DOI=10.1128/JVI.73.1.251-259.1999; PMCID=PMC103829
Ursula J. Buchholz, Stefan Finke, Karl-Klaus Conzelmann;
Generation of bovine respiratory syncytial virus (BRSV) from cDNA: BRSV NS2 is not essential for virus replication in tissue culture, and the human RSV leader region acts as a functional BRSV genome promoter.
J. Virol. 73:251-259(1999)

Cross-references
Cell line collections (Providers) CCLV; CCLV-RIE 0583
Encyclopedic resources Wikidata; Q54798410
Entry history
Entry creation05-Mar-2018
Last entry update14-Aug-2025
Version number10