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Cellosaurus FT33-shp53-R24C (CVCL_RK66)

[Text version]
Cell line name FT33-shp53-R24C
Synonyms FT33; iFTSEC33
Accession CVCL_RK66
Resource Identification Initiative To cite this cell line use: FT33-shp53-R24C (RRID:CVCL_RK66)
Comments Knockout cell: Method=shRNA knockdown; HGNC; HGNC:11998; TP53.
Genetic integration: Method=Transduction; Gene=HGNC; HGNC:1773; CDK4 (Note=With p.Arg24Cys).
Genetic integration: Method=Transduction; Gene=HGNC; HGNC:11730; TERT.
Genetic integration: Method=Transduction; Gene=UniProtKB; P00557; Escherichia coli hygromycin-B 4-O-kinase (hph) (HygR).
Genetic integration: Method=Transduction; Gene=UniProtKB; P00552; Klebsiella pneumoniae transposon Tn5 neo.
Omics: Genomics; ChIP-seq; H3K27ac.
Omics: Genomics; ChIP-seq; H3K4me1.
Derived from site: In situ; Fallopian tube; UBERON=UBERON_0003889.
Cell type: Fallopian tube secretory epithelial cell; CL=CL_4030006.
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Sex of cell Female
Age at sampling Age unspecified
Category Telomerase immortalized cell line
Publications

PubMed=21502498; DOI=10.1073/pnas.1017300108; PMCID=PMC3088633
Alison M. Karst, Keren Levanon, Ronny I. Drapkin;
Modeling high-grade serous ovarian carcinogenesis from the fallopian tube.
Proc. Natl. Acad. Sci. U.S.A. 108:7547-7552(2011)

PubMed=22936217; DOI=10.1038/nprot.2012.097; PMCID=PMC7433321
Alison M. Karst, Ronny I. Drapkin;
Primary culture and immortalization of human fallopian tube secretory epithelial cells.
Nat. Protoc. 7:1755-1764(2012)

PubMed=25804953; DOI=10.1093/hmg/ddv101; PMCID=PMC4459387
Simon Gert Coetzee, Howard C. Shen, Dennis J. Hazelett, Kate Lawrenson, Karoline Kuchenbaecker, Jonathan P. Tyrer, Suhn K. Rhie, Keren Levanon, Alison M. Karst, Ronny I. Drapkin, Susan J. Ramus ...Show all 21 authors... , Fergus J. Couch, Kenneth Offit, Georgia Chenevix-Trench, Alvaro N.A. Monteiro, Antonis Antoniou, Matthew Lawrence Freedman, Gerhard A. Coetzee, Paul D.P. Pharoah, Houtan Noushmehr, Simon Andrew Gayther; Show fewer authors
Consortium of Investigators of Modifiers of BRCA1/2
Ovarian Cancer Association Consortium
Cell-type-specific enrichment of risk-associated regulatory elements at ovarian cancer susceptibility loci.
Hum. Mol. Genet. 24:3595-3607(2015)

PubMed=30487138; DOI=10.1158/0008-5472.CAN-17-3864; PMCID=PMC6359979
Melissa A. Buckley, Nicholas T. Woods, Jonathan P. Tyrer, Gustavo Mendoza-Fandino, Kate Lawrenson, Dennis J. Hazelett, Hamed S. Najafabadi, Anxhela Gjyshi, Renato S. Carvalho, Paulo C. Lyra Jr., Simon Gert Coetzee ...Show all 27 authors... , Howard C. Shen, Ally W. Yang, Madalene A. Earp, Sean J. Yoder, Harvey Risch, Georgia Chenevix-Trench, Susan J. Ramus, Catherine M. Phelan, Gerhard A. Coetzee, Houtan Noushmehr, Timothy R. Hughes, Thomas A. Sellers, Ellen L. Goode, Paul D.P. Pharoah, Simon Andrew Gayther, Alvaro N.A. Monteiro; Show fewer authors
Functional analysis and fine mapping of the 9p22.2 ovarian cancer susceptibility locus.
Cancer Res. 79:467-481(2019)

Cross-references
Cell line collections (Providers) ABM; T0609 - Discontinued
Encyclopedic resources Wikidata; Q54835216
Gene expression databases GEO; GSM1663024
GEO; GSM1663025
GEO; GSM1663026
GEO; GSM1663027
GEO; GSM1663028
GEO; GSM1663029
GEO; GSM1663030
GEO; GSM1663031
Entry history
Entry creation05-Mar-2018
Last entry update14-Aug-2025
Version number13