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Cellosaurus HCT 116 CMV-OsTIR1 (CVCL_RJ11)

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Cell line name HCT 116 CMV-OsTIR1
Synonyms HCT116 CMV-OsTIR1
Accession CVCL_RJ11
Resource Identification Initiative To cite this cell line use: HCT 116 CMV-OsTIR1 (RRID:CVCL_RJ11)
Comments Population: Caucasian.
Characteristics: Parent cell line to study the rapid depletion of a protein. The expressed rice TIR1 can form a functional SCF(OsTIR1) E3 ligase complex with the endogenous components in human cells. In the presence of auxin, a protein of interest fused with mAID is rapidly degraded upon poly-ubiquitylation.
Characteristics: Using CRISPR/Cas9 a CMV-OsTIR1-PuroR (pMK232) vector was introduced in both alleles of the AAVS1 safe harbor locus.
Genetic integration: Method=CRISPR/Cas9; Gene=UniProtKB; Q7XVM8; Oryza sativa subsp. japonica TIR1-like protein Os04g0395600.
Genetic integration: Method=CRISPR/Cas9; Gene=UniProtKB; P13249; Streptomyces alboniger pac (PuroR).
Derived from site: In situ; Colon; UBERON=UBERON_0001155.
Sequence variations
  • Mutation; HGNC; HGNC:173; ACVR2A; Simple; p.Lys437Argfs*5 (c.1310delA); dbSNP=rs764719749; Zygosity=Homozygous (from parent cell line).
  • Mutation; HGNC; HGNC:1101; BRCA2; Simple; p.Ile2675Aspfs*6 (c.8021dupA) (c.8021_8022insA); ClinVar=VCV000267050; Zygosity=Heterozygous (from parent cell line).
  • Mutation; HGNC; HGNC:1787; CDKN2A; Simple; p.Arg24Serfs*20 (c.68dupG) (c.68_69insG) (p.G23fs); Zygosity=Heterozygous (from parent cell line).
  • Mutation; HGNC; HGNC:2514; CTNNB1; Simple; p.Ser45del (c.133_135delTCT); ClinVar=VCV000017576; Zygosity=Heterozygous (from parent cell line).
  • Mutation; HGNC; HGNC:3373; EP300; Simple; p.Met1470Cysfs*22 (c.4408delA); Zygosity=Heterozygous (from parent cell line).
  • Mutation; HGNC; HGNC:3373; EP300; Simple; p.Asn1700Thrfs*9 (c.5099delA); Zygosity=Heterozygous (from parent cell line).
  • Mutation; HGNC; HGNC:6407; KRAS; Simple; p.Gly13Asp (c.38G>A); ClinVar=VCV000012580; Zygosity=Heterozygous (from parent cell line).
  • Mutation; HGNC; HGNC:8975; PIK3CA; Simple; p.His1047Arg (c.3140A>G); ClinVar=VCV000013652; Zygosity=Heterozygous (from parent cell line).
  • Mutation; HGNC; HGNC:9277; PPM1D; Simple; p.Leu450Ter (c.1349delT) (p.Leu450fs) (c.1344delT); Zygosity=Heterozygous (from parent cell line).
  • Mutation; HGNC; HGNC:11773; TGFBR2; Simple; p.Lys128Serfs*35 (c.383delA); ClinVar=VCV000477546; Zygosity=Homozygous (from parent cell line).
Disease Colon carcinoma (NCIt: C4910)
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_0291 (HCT 116)
Sex of cell Male
Age at sampling 48Y
Category Cancer cell line
Publications

PubMed=27052166; DOI=10.1016/j.celrep.2016.03.001
Toyoaki Natsume, Tomomi Kiyomitsu, Yumiko Saga, Masato T. Kanemaki;
Rapid protein depletion in human cells by auxin-inducible degron tagging with short homology donors.
Cell Rep. 15:210-218(2016)

Cross-references
Cell line collections (Providers) RCB; RCB4662
Cell line databases/resources cancercelllines; CVCL_RJ11
Encyclopedic resources Wikidata; Q54881927
Entry history
Entry creation05-Mar-2018
Last entry update14-Aug-2025
Version number14