Cellosaurus HCT116-Fluc-Neo/eGFP-Puro (CVCL_RA38)
Cell line name | HCT116-Fluc-Neo/eGFP-Puro |
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Accession | CVCL_RA38 |
Resource Identification Initiative | To cite this cell line use: HCT116-Fluc-Neo/eGFP-Puro (RRID:CVCL_RA38) |
Comments | Population: Caucasian. Characteristics: Reporter cell line, transfected with both the LV-Fluc-P2A-Neo and LV-eGFP-PGK-Puro vectors. LV-Fluc-P2A-Neo encodes for luciferase linked via a P2A cleavage peptide to the neo gene and under control of the spleen focus-forming virus (SFFV) promoter. LV-eGFP-PGK-Puro encodes for eGFP under control of the spleen focus-forming virus (SFFV) promoter and the pac gene under the control of the mouse PGK promoter. Transfected with: UniProtKB; P42212; GFP (with p.Phe64Leu, p.Ser65Thr and p.His231Leu = EGFP). Transfected with: UniProtKB; P08659; Firefly luciferase. Transfected with: UniProtKB; P00552; Transposon Tn5 neo. Transfected with: UniProtKB; P13249; S.alboniger pac (PuroR). Derived from site: In situ; Colon; UBERON=UBERON_0001155. |
Sequence variations |
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Disease | Colon carcinoma (NCIt: C4910) |
Species of origin | Homo sapiens (Human) (NCBI Taxonomy: 9606) |
Hierarchy | Parent: CVCL_0291 (HCT 116) |
Sex of cell | Male |
Age at sampling | 48Y |
Category | Cancer cell line |
Cross-references | |
Cell line collections (Providers) | Imanis; CL070 |
Cell line databases/resources | cancercelllines; CVCL_RA38 |
Encyclopedic resources | Wikidata; Q54882036 |
Entry history | |
Entry creation | 05-Mar-2018 |
Last entry update | 05-Oct-2023 |
Version number | 11 |