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Cellosaurus HEK293T SMARCB1-/- (CVCL_QW54)

[Text version]
Cell line name HEK293T SMARCB1-/-
Synonyms SMARCB1-/- HEK293T; BAF47-knockout HEK293T
Accession CVCL_QW54
Resource Identification Initiative To cite this cell line use: HEK293T SMARCB1-/- (RRID:CVCL_QW54)
Comments Knockout cell: Method=CRISPR/Cas9; HGNC; HGNC:11103; SMARCB1.
Genetic integration: Method=Transfection; Gene=UniProtKB; P00552; Klebsiella pneumoniae transposon Tn5 neo.
Genetic integration: Method=Transfection; Gene=UniProtKB; P03070; Simian virus 40 (SV40) large T antigen.
Transformant: NCBI_TaxID; 28285; Human adenovirus C serotype 5.
Transformant: NCBI_TaxID; 1891767; Simian virus 40 (SV40) (Note=tsA).
Derived from site: In situ; Fetal kidney; UBERON=UBERON_0002113.
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_0063 (HEK293T)
Sex of cell Female
Age at sampling Fetus
Category Transformed cell line
Publications

PubMed=28945250; DOI=10.1038/ng.3958; PMCID=PMC5803080
Robert T. Nakayama, John L. Pulice, Alfredo M. Valencia, Matthew J. McBride, Zachary M. McKenzie, Mark A. Gillespie, Wai Lim Ku, Ming-Xiang Teng, Kai-Rong Cui, Robert T. Williams, Seth H. Cassel ...Show all 18 authors... , He Qing, Christian J. Widmer, George Daniel Demetri, Rafael Angel Irizarry, Ke-Ji Zhao, Jeffrey A. Ranish, Cigall Kadoch; Show fewer authors
SMARCB1 is required for widespread BAF complex-mediated activation of enhancers and bivalent promoters.
Nat. Genet. 49:1613-1623(2017)

Cross-references
Encyclopedic resources Wikidata; Q54882490
Entry history
Entry creation15-Nov-2017
Last entry update14-Aug-2025
Version number10