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Cellosaurus MMNK-1 (CVCL_M266)

[Text version]
Cell line name MMNK-1
Synonyms MMNK1
Accession CVCL_M266
Resource Identification Initiative To cite this cell line use: MMNK-1 (RRID:CVCL_M266)
Comments Group: Patented cell line.
Registration: International Depositary Authority, Japanese National Institute of Bioscience and Human-Technology, Agency of Industrial Science and Technology; FERM BP-8126.
Population: Japanese.
Characteristics: Because it has been transduced by a construct containing LoxP sites, transfection with the Cre recombinase eliminates the SV40 T gene thus reverting the cells to a non-immortal phenotype.
Characteristics: Transduced with a retroviral vector SSR#197 (MoMLV/LTR-LoxP-hTERT-IRES-EGFP-LoxP-MoMLV/LTR) expressing hTERT and eGFP flanked by a pair of loxP sites.
Genetic integration: Method=Transduction; Gene=FPbase; R9NL8; eGFP (Note=Enhanced GFP).
Genetic integration: Method=Transduction; Gene=HGNC; HGNC:11730; TERT.
Genetic integration: Method=Transfection; Gene=UniProtKB; P03070; SV40 large T antigen.
Genetic integration: Method=Transfection; Gene=UniProtKB; P00552; Transposon Tn5 neo.
Transformant: NCBI_TaxID; 1891767; Simian virus 40 (SV40) (Note=pSV3-neo).
Derived from site: In situ; Fetal liver; UBERON=UBERON_0002107.
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_9131 (OUMS-21 [1993])
Children:
CVCL_AE16 (oxMMNK1-L)
Sex of cell Sex unspecified
Age at sampling 18FW
Category Telomerase immortalized cell line
STR profile Source(s): JCRB=JCRB1554

Markers:
AmelogeninX
CSF1PO11,12
D5S81811,15
D7S8208,9
D13S3179,12
D16S53911,13
TH017,9
TPOX8,11
vWA14,17

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Publications

PubMed=14966424; DOI=10.1097/01.TP.0000110292.73873.25
Maruyama M., Kobayashi N., Westerman K.A., Sakaguchi M., Allain J.-E., Totsugawa T., Okitsu T., Fukazawa T., Weber A., Stolz D.B., Leboulch P., Tanaka N.
Establishment of a highly differentiated immortalized human cholangiocyte cell line with SV40T and hTERT.
Transplantation 77:446-451(2004)

PubMed=21871105; DOI=10.1186/1476-4598-10-102; PMCID=PMC3173387
Obchoei S., Weakley S.M., Wongkham S., Wongkham C., Sawanyawisuth K., Yao Q.-Z., Chen C.-Y.
Cyclophilin A enhances cell proliferation and tumor growth of liver fluke-associated cholangiocarcinoma.
Mol. Cancer 10:102.1-102.15(2011)

PubMed=27231123; DOI=10.1158/2159-8290.CD-15-1442; PMCID=PMC5458737
Saha S.K., Gordan J.D., Kleinstiver B.P., Vu P., Najem M.S., Yeo J.-C., Shi L., Kato Y., Levin R.S., Webber J.T., Damon L.J., Egan R.K., Greninger P., McDermott U., Garnett M.J., Jenkins R.L., Rieger-Christ K.M., Sullivan T.B., Hezel A.F., Liss A.S., Mizukami Y., Goyal L., Ferrone C.R., Zhu A.X.-X., Joung J.K., Shokat K.M., Benes C.H., Bardeesy N.M.
Isocitrate dehydrogenase mutations confer dasatinib hypersensitivity and SRC dependence in intrahepatic cholangiocarcinoma.
Cancer Discov. 6:727-739(2016)

Cross-references
Cell line collections (Providers) JCRB; JCRB1554
Encyclopedic resources Wikidata; Q54906231
Entry history
Entry creation05-Nov-2013
Last entry update19-Dec-2024
Version number22