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Cellosaurus MMNK-1 (CVCL_M266)

[Text version]
Cell line name MMNK-1
Synonyms MMNK1
Accession CVCL_M266
Resource Identification Initiative To cite this cell line use: MMNK-1 (RRID:CVCL_M266)
Comments Group: Patented cell line.
Registration: International Depositary Authority, Japanese National Institute of Bioscience and Human-Technology, Agency of Industrial Science and Technology; FERM BP-8126.
Population: Japanese.
Characteristics: Because it has been transduced by a construct containing LoxP sites, transfection with the Cre recombinase eliminates the SV40 T gene thus reverting the cells to a non-immortal phenotype.
Characteristics: Transduced with a retroviral vector SSR#197 (MoMLV/LTR-LoxP-hTERT-IRES-EGFP-LoxP-MoMLV/LTR) expressing hTERT and eGFP flanked by a pair of loxP sites.
Transfected with: HGNC; 11730; TERT.
Transfected with: UniProtKB; P42212; GFP (with p.Phe64Leu, p.Ser65Thr and p.His231Leu = EGFP).
Transfected with: UniProtKB; P00552; Transposon Tn5 neo.
Transformant: NCBI_TaxID; 1891767; Simian virus 40 (SV40) [pSV3-neo].
Derived from sampling site: Fetal liver. Cell type=Cholangiocyte.
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_9131 (OUMS-21 [1993])
Sex of cell Sex unspecified
Age at sampling 18FW
Category Telomerase immortalized cell line
STR profile Source(s): JCRB


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PubMed=14966424; DOI=10.1097/01.TP.0000110292.73873.25
Maruyama M., Kobayashi N., Westerman K.A., Sakaguchi M., Allain J.-E., Totsugawa T., Okitsu T., Fukazawa T., Weber A., Stolz D.B., Leboulch P., Tanaka N.
Establishment of a highly differentiated immortalized human cholangiocyte cell line with SV40T and hTERT.
Transplantation 77:446-451(2004)

PubMed=21871105; DOI=10.1186/1476-4598-10-102
Obchoei S., Weakley S.M., Wongkham S., Wongkham C., Sawanyawisuth K., Yao Q., Chen C.
Cyclophilin A enhances cell proliferation and tumor growth of liver fluke-associated cholangiocarcinoma.
Mol. Cancer 10:102.1-102.15(2011)

PubMed=27231123; DOI=10.1158/2159-8290.CD-15-1442
Saha S.K., Gordan J.D., Kleinstiver B.P., Vu P., Najem M.S., Yeo J.-C., Shi L., Kato Y., Levin R.S., Webber J.T., Damon L.J., Egan R.K., Greninger P., McDermott U., Garnett M.J., Jenkins R.L., Rieger-Christ K.M., Sullivan T.B., Hezel A.F., Liss A.S., Mizukami Y., Goyal L., Ferrone C.R., Zhu A.X., Joung J.K., Shokat K.M., Benes C.H., Bardeesy N.
Isocitrate dehydrogenase mutations confer dasatinib hypersensitivity and SRC dependence in intrahepatic cholangiocarcinoma.
Cancer Discov. 6:727-739(2016)

Cell line collections JCRB; JCRB1554
Encyclopedic resources Wikidata; Q54906231
Entry history
Entry creation05-Nov-2013
Last entry update21-Mar-2023
Version number17