Cellosaurus cell line CHLA-25 (CVCL

Cellosaurus CHLA-25 (CVCL_M152)

[Text version]

Cell line name

CHLA-25

Synonyms

CHLA25

Accession

CVCL_M152

Resource Identification Initiative

To cite this cell line use: CHLA-25 (RRID:CVCL_M152)

Comments

Part of: Ewing Sarcoma Cell Line Atlas (ESCLA).
Doubling time: 99 hours (PubMed=24312454).
Omics: Genomics; ChIP-seq; ERG.
Omics: Genomics; ChIP-seq; H3K27ac.
Omics: Genomics; ChIP-seq; H3K27me3.
Omics: Genomics; ChIP-seq; H3K4me3.
Omics: Genomics; DNA methylation analysis.
Omics: Genomics; Whole genome sequencing.
Omics: Phenotyping; Drug screening.
Omics: Proteomics.
Omics: Transcriptomics; RNAseq.

Sequence variations

Gene fusion; HGNC; HGNC:3446; ERG + HGNC; HGNC:3508; EWSR1; Name(s)=EWSR1-ERG, EWS-ERG; Note=EWSR1 exon 7 with ERG exon 6 (PubMed=25010205; PubMed=36476851).
Mutation; HGNC; HGNC:11998; TP53; Simple; p.Arg175His (c.524G>A); ClinVar=VCV000012374; Zygosity=Unspecified (PubMed=25010205).

Disease

Primitive neuroectodermal tumor (NCIt: C3716)

Species of origin

Homo sapiens (Human) (NCBI Taxonomy: 9606)

Originate from same individual

CVCL_A444 ! TTC-466

Sex of cell

Female

Age at sampling

2Y7M

Category

Cancer cell line

STR profile

Source(s): COG; PubMed=24312454; PubMed=25010205

Markers:

Amelogenin	X
CSF1PO	10
D2S1338	19,22
D3S1358	17,18
D5S818	10
D7S820	8,10
D8S1179	13,15
D13S317	10,12
D16S539	11,12
D18S51	14,17
D19S433	14
D21S11	29,32.2
FGA	24,25
TH01	7
TPOX	8
vWA	15,17

Web pages

Info; ESCLA; -; https://hgserver1.amc.nl/cgi-bin/r2/main.cgi?option=about_dscope
Provider; CCR; -; https://www.cccells.org/PDF_Files/Ewings/CHLA-25%20Cell%20Line%20Data%20Sheet.pdf
Provider; CCR; -; https://www.cccells.org/tables/Ewings.php

Publications

PubMed=24312454; DOI=10.1371/journal.pone.0080060; PMCID=PMC3846563
William A. May, Rita S. Grigoryan, Nino Keshelava, Daniel J. Cabral, Laura L. Christensen, Jasmine Jenabi, Ling-Yun Ji, Timothy J. Triche, Elizabeth R. Lawlor, Charles Patrick Reynolds;
Characterization and drug resistance patterns of Ewing's sarcoma family tumor cell lines.
PLoS ONE 8:e80060.1-e80060.10(2013)

PubMed=25010205; DOI=10.1371/journal.pgen.1004475; PMCID=PMC4091782
Andrew S. Brohl, David A. Solomon, Wendy Chang, Jian-Jun Wang, Young Song, Sivasish Sindiri, Rajesh Patidar, Laura Hurd, Li Chen, Jack F. Shern, Hong-Ling Liao ...Show all 26 authors... , Xin-Yu Wen, Julia Gerard, Jung-Sik Kim, Jose Antonio Lopez Guerrero, Isidro Machado, Daniel H. Wai, Piero Picci, Timothy J. Triche, Andrew E. Horvai, Markku Martti Miettinen, Jun S. Wei, Daniel Catchpoole, Antonio Llombart-Bosch, Todd A. Waldman, Javed Khan; Show fewer authors
The genomic landscape of the Ewing sarcoma family of tumors reveals recurrent STAG2 mutation.
PLoS Genet. 10:e1004475.1-e1004475.13(2014)

PubMed=26351324; DOI=10.1158/1535-7163.MCT-15-0074; PMCID=PMC4636476
Beverly A. Teicher, Eric C. Polley, Mark W. Kunkel, David Evans, Thomas E. Silvers, Rene M. Delosh, Julie Laudeman, Chad Ogle, Russell Reinhart, Michael Selby, John Connelly ...Show all 14 authors... , Erik Harris, Anne Monks, Joel Morris; Show fewer authors
Sarcoma cell line screen of oncology drugs and investigational agents identifies patterns associated with gene and microRNA expression.
Mol. Cancer Ther. 14:2452-2462(2015)

DOI=10.5282/edoc.27750
Martin Franz Orth;
Systematic multi-omics profiling of Ewing sarcoma cell lines.
Thesis PhD (2021); Ludwig Maximilians University of Munich; Munich; Germany

PubMed=36476851; DOI=10.1016/j.celrep.2022.111761; PMCID=PMC10333306
Martin Franz Orth, Didier Surdez, Tobias Faehling, Anna C. Ehlers, Aruna Marchetto, Sandrine Grossetete-Lalami, Richard Volckmann, Danny A. Zwijnenburg, Julia Sophie Gerke, Sakina Zaidi, Javier Alonso ...Show all 25 authors... , Ana Sastre, Sylvain Baulande, Martin Sill, Florencia Cidre-Aranaz, Shunya Ohmura, Thomas Kirchner, Stefanie M. Hauck, Eva Reischl, Melissa Gymrek, Stefan Michael Pfister, Konstantin Strauch, Jan Koster, Olivier Delattre, Thomas Georg Philipp Grunewald; Show fewer authors
Systematic multi-omics cell line profiling uncovers principles of Ewing sarcoma fusion oncogene-mediated gene regulation.
Cell Rep. 41:111761.1-111761.28(2022)

Cross-references

Cell line databases/resources

cancercelllines; CVCL_M152

Encyclopedic resources

Wikidata; Q54812489

Gene expression databases

GEO; GSM1676353
GEO; GSM1701683
GEO; GSM5359640
GEO; GSM5359641
GEO; GSM5359642
GEO; GSM5359643
GEO; GSM5359644
GEO; GSM5359645
GEO; GSM5362884
GEO; GSM5362885
GEO; GSM5362886
GEO; GSM5362887
GEO; GSM5362888
GEO; GSM5362889
GEO; GSM5363946
GEO; GSM5363947
GEO; GSM5363948
GEO; GSM5363949
GEO; GSM5363950

Polymorphism and mutation databases

Cosmic; 2228216

Entry history

Entry creation

06-May-2013

Last entry update

10-Apr-2025

Version number