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Cellosaurus UHi006-A-1 (CVCL_LJ89)

[Text version]
Cell line name UHi006-A-1
Synonyms HEL24.3-SOX2-NTdT; HEL24.3-SOX2-NTdT-C9-H5; UHi001-A
Accession CVCL_LJ89
Resource Identification Initiative To cite this cell line use: UHi006-A-1 (RRID:CVCL_LJ89)
Comments From: University of Helsinki; Helsinki; Finland.
Population: Caucasian.
Characteristics: Expresses bright nuclear located tDTomato upon SOX2 activation. Heterozygous knockin of a T2A-NLS-tdTomato-F2A-Pac cassette at the C-terminus of the SOX2 gene by CRISPR/Cas9.
Genetic integration: Method=Transfection/transduction; Gene=FPbase; PGG5S; tdTomato (Note=Bright red fluorescent protein derived from drFP583 (DsRed)).
Genetic integration: Method=Transfection/transduction; Gene=UniProtKB; P13249; S.alboniger pac (PuroR).
Derived from site: In situ; Foreskin, skin; UBERON=UBERON_0001471.
Cell type: Fibroblast of foreskin; CL=CL_1001608.
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_9T96 (UHi006-A)
Sex of cell Male
Age at sampling <1M
Category Induced pluripotent stem cell
Publications

PubMed=28952927; DOI=10.1016/j.scr.2017.05.005
Balboa D., Weltner J., Novik Y., Eurola S., Wartiovaara K., Otonkoski T.
Generation of a SOX2 reporter human induced pluripotent stem cell line using CRISPR/SaCas9.
Stem Cell Res. 22:16-19(2017)

Cross-references
Cell line databases/resources hPSCreg; UHi006-A-1
Biological sample resources BioSamples; SAMEA104134270
Encyclopedic resources Wikidata; Q54990146
Entry history
Entry creation15-Nov-2017
Last entry update10-Sep-2024
Version number11