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Cellosaurus UHi006-A-2 (CVCL_LJ88)

[Text version]
Cell line name UHi006-A-2
Synonyms HEL24.3-OCT4-nEmGFP; HEL24.3-OCT4-nEmGFP-A-G3; UHi001-B
Accession CVCL_LJ88
Resource Identification Initiative To cite this cell line use: UHi006-A-2 (RRID:CVCL_LJ88)
Comments From: University of Helsinki; Helsinki; Finland.
Population: Caucasian.
Characteristics: Expresses bright nuclear located EmGFP upon POU5F1 activation. Heterozygous knockin by CRISPR/Cas9 of a T2A-NLS-EmGFP-P2A-Pac cassette at the C-terminus of the POU5F1/OCT4 gene by CRISPR/Cas9.
Genetic integration: Method=CRISPR/Cas9; Gene=FPbase; GLAD6; Emerald (Note=EmGFP, green fluorescent protein derived from GFP).
Genetic integration: Method=CRISPR/Cas9; Gene=UniProtKB; P13249; Streptomyces alboniger pac (PuroR).
Derived from site: In situ; Foreskin, skin; UBERON=UBERON_0001471.
Cell type: Fibroblast of foreskin; CL=CL_1001608.
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_9T96 (UHi006-A)
Sex of cell Male
Age at sampling <1M
Category Induced pluripotent stem cell
STR profile Source(s): PubMed=28925359

Markers:
AmelogeninX,Y
CSF1PO10
D5S81811,12
D7S8208,10
D13S31712
D16S53911,12
D21S1129
TH017,9.3
TPOX11
vWA16,18

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Publications

PubMed=28925359; DOI=10.1016/j.scr.2017.07.006
Diego Balboa, Jere Weltner, Yuval Novik, Solja Eurola, Kirmo Wartiovaara, Timo Otonkoski;
Generation of an OCT4 reporter human induced pluripotent stem cell line using CRISPR/SpCas9.
Stem Cell Res. 23:105-108(2017)

Cross-references
Cell line databases/resources hPSCreg; UHi006-A-2
Biological sample resources BioSamples; SAMEA104277230
Encyclopedic resources Wikidata; Q54990148
Entry history
Entry creation15-Nov-2017
Last entry update14-Aug-2025
Version number13