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Cellosaurus MB1.3.19 (CVCL_LI59)

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Cell line name MB1.3.19
Accession CVCL_LI59
Resource Identification Initiative To cite this cell line use: MB1.3.19 (RRID:CVCL_LI59)
Comments Genetic integration: Method=Transfection; Gene=HGNC; HGNC:5173; HRAS (Note=With p.Gly12Lys).
Genetic integration: Method=Transfection; Gene=UniProtKB; P00552; Klebsiella pneumoniae transposon Tn5 neo.
Genetic integration: Method=Transfection; Gene=UniProtKB; P03070; Simian virus 40 (SV40) large T antigen.
Transformant: NCBI_TaxID; 1891767; Simian virus 40 (SV40).
Derived from site: In situ; Embryonic brown adipose tissue; UBERON=UBERON_0001348.
Cell type: Preadipocyte; CL=CL_0002334.
Species of origin Rattus norvegicus (Rat) (NCBI Taxonomy: 10116)
Breed/subspecies: Wistar; RS=RS_0000955.
Sex of cell Sex unspecified
Age at sampling 20FD
Category Transformed cell line
Publications

PubMed=8262142; DOI=10.1006/excr.1993.1308
Manuel Benito, Almudena Porras, Eugenio Santos;
Establishment of permanent brown adipocyte cell lines achieved by transfection with SV40 large T antigen and ras genes.
Exp. Cell Res. 209:248-254(1993)

PubMed=8877105
Margarita Lorenzo, Angela Maria Valverde, Teresa Teruel, Alberto Alvarez, Manuel Benito;
p21ras induced differentiation-related gene expression in fetal brown adipocyte primary cells and cell lines.
Cell Growth Differ. 7:1251-1259(1996)

Cross-references
Encyclopedic resources Wikidata; Q54904100
Entry history
Entry creation15-Nov-2017
Last entry update14-Aug-2025
Version number10