Cellosaurus (CVCL_KY11)
| Comments | Characteristics: Using homologous recombination the Sox1 reading frame was replaced by a GFP-IRES-PuroR construct. Expresses eGFP under the control of the Sox-1 promoter (from parent cell line). Doubling time: ~24 hours (PubMed=16086633). Selected for resistance to: ChEBI; CHEBI_9555; Tioguanine (6-thioguanine; 6-TG). Genetic integration: Method=Homologous recombination; Gene=FPbase; R9NL8; eGFP (Note=Enhanced GFP). Genetic integration: Method=Homologous recombination; Gene=UniProtKB; P13249; Streptomyces alboniger pac (PuroR). Derived from site: In situ; Blastocyst; UBERON=UBERON_0000358. Cell type: Neural stem cell; CL=CL_0000047. |
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| Species of origin | Cricetulus griseus (Chinese hamster) (Cricetulus barabensis griseus)
(NCBI Taxonomy: 10090)
Breed/subspecies: 129P2/Ola. |
| Hierarchy | Parent: CVCL_Y482 (46C) |
| Sex of cell | Male |
| Age at sampling | Blastocyst stage |
| Category | Somatic stem cell |
| Entry history | |
| Entry creation | 20-May-2021 |
| Last entry update | 14-Aug-2025 |
| Version number | 10 |