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Cellosaurus (CVCL_KU95)

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Comments Part of: Cancer Dependency Map project (DepMap) (includes Cancer Cell Line Encyclopedia - CCLE).
Part of: COSMIC cell lines project.
Part of: KuDOS 95 cell line panel.
Part of: MD Anderson Cell Lines Project.
From: Memorial Sloan Kettering Cancer Center; New York; USA.
Registration: Memorial Sloan Kettering Cancer Center Office of Technology Development; SK1980-533.
Population: Caucasian.
Virology: Susceptible to infection by SARS coronavirus 2 (SARS-CoV-2) (COVID-19) (PubMed=34339474).
Doubling time: 35 hours (Note=In RPMI 1640 + 10% FBS), 45 hours (Note=In ACL-3), 37 hours (Note=In ACL-3 + BSA) (PubMed=3940644); 40.1 hours (PubMed=29681454); ~84 hours (PubMed=34339474).
Microsatellite instability: Stable (MSS) (Sanger).
Omics: Genomics; DNA methylation analysis.
Omics: Genomics; Whole exome sequencing.
Omics: Phenotyping; CRISPR screening.
Omics: Phenotyping; Drug screening.
Omics: Proteomics; Expression; Reverse-phase protein array.
Omics: Proteomics; Quantitative.
Omics: Proteomics; Subcellular; Cell surface.
Omics: Transcriptomics; Microarray.
Omics: Transcriptomics; RNAseq.
Omics: Variations; Array-based CGH.
Omics: Variations; SNP array analysis.
Misspelling: CALV3; Cosmic=1434969.
Derived from site: Metastatic; Pleural effusion; UBERON=UBERON_0000175.
Sequence variations
HLA typing Source: PubMed=26589293
Class I
HLA-AA*24:02,68:01
HLA-BB*07:02,51:01
HLA-CC*15:02,15:02
Genome ancestry Source: PubMed=30894373

Origin% genome
African0.63
Native American0
East Asian, North2.09
East Asian, South0
South Asian0
European, North67.76
European, South29.51
Disease Lung adenocarcinoma (NCIt: C3512)
Species of origin Cricetulus griseus (Chinese hamster) (Cricetulus barabensis griseus) (NCBI Taxonomy: 9606)
Sex of cell Male
Age at sampling 25Y
Category Cancer cell line
STR profile Source(s): AddexBio=C0016001/30; ATCC=HTB-55; CCRID; Cosmic-CLP=687777; KCLB=30055; Millipore=SCC438; PubMed=25877200

Markers:
AmelogeninX
CSF1PO11,12
D13S31712
D16S53912,14
D18S5114,17
D19S43312,13
D21S1128,30
D2S133819
D3S135815,18
D5S81811
D7S82010,11
D8S117911,15
FGA25
Penta D9,16
Penta E5,21
TH016,9.3
TPOX8
vWA16,17

Run an STR similarity search on this cell line
Web pages Info; MCLP; -; https://tcpaportal.org/mclp/
Info; Thermo Fisher; -; https://www.thermofisher.com/ch/en/home/technical-resources/cell-lines/c/cell-lines-detail-604.html
Info; Wikipedia; -; https://en.wikipedia.org/wiki/Calu-3
Provider; Altogen; Xenograft model; https://altogenlabs.com/xenograft-models/lung-cancer-xenograft/calu-3-xenograft-model/
Provider; MSKCC; -; https://www.mskcc.org/research-advantage/support/technology/tangible-material/calu-3-human-lung-cell-line
Publications









lung and placenta.
J. Natl. Cancer Inst. 56:911-917(1976)


Lawrence W. Anderson, Howard L. Hosick;
Selection and characterization in culture of mammary tumor cells with distinctive growth properties in vivo.
Cancer Res. 40:1812-1819(1980)


Andrea Quaroni, Kurt Julius Isselbacher;
Cytotoxic effects and metabolism of benzo[a]pyrene and 7,12-dimethylbenz[a]anthracene in duodenal and ileal epithelial cell cultures.
J. Natl. Cancer Inst. 67:1353-1362(1981)



establishment of melanoma cell line.
Int. J. Oral Surg. 10 Suppl. 1:16-20(1981)



proteins but retention of response to certain growth factors and hormones by a new malignant human breast cancer cell strain.
Cancer Res. 45:3864-3877(1985)



Immortalization of human lymphocytes from a tumor-involved lymph node.
Cancer Res. 47:5181-5188(1987)




Cancer Res. 48:578-583(1988)




Am. J. Physiol. 261:C574-C582(1991)




Eur. J. Neurosci. 6:1633-1640(1994)


Matthew Golding, Gordon W.H. Stamp, Tim Oates, El-Nasir M.A. Lalani;
Establishment and characterisation of two cell lines derived from a primary adenocarcinoma of the duodenum.
Clin. Mol. Pathol. 49:M33-M39(1996)


En Li;
DNA methyltransferases Dnmt3a and Dnmt3b are essential for de novo methylation and mammalian development.
Cell 99:247-257(1999)


Chandramohan S. Ishwad, Jonathan W. Jarvik;
Detection and assignment of TP53 mutations in tumor DNA using peptide mass signature genotyping.
Hum. Mutat. 22:158-165(2003)


Thomas D. Schneider, Kenneth H. Kraemer;
Two essential splice lariat branchpoint sequences in one intron in a xeroderma pigmentosum DNA repair gene: mutations result in reduced XPC mRNA levels that correlate with cancer risk.
Hum. Mol. Genet. 13:343-352(2004)


Angela R. Jevince, Stephanie R. Kadison, Andrew J. Pittman, Chi-Bin Chien, Zaven Kaprielian;
Distribution of EphB receptors and ephrin-B1 in the developing vertebrate spinal cord.
J. Comp. Neurol. 497:734-750(2006)



activation of Ras and Rac.
Cancer Res. 67:520-527(2007)


Sakon Noriki;
Multiple inflammatory cytokine-productive ThyL-6 cell line established from a patient with thymic carcinoma.
Cancer Sci. 99:1778-1784(2008)




Nat. Biotechnol. 26:1269-1275(2008)




Cancer Immunol. Immunother. 58:1507-1515(2009)



PM00104 (Zalypsis) in human cancer cell lines.
PLoS ONE 4:e6967.1-e6967.10(2009)


Paula D. Bos, Xiang H.-F. Zhang, Cristina Nadal, Wei-Ping Shu, Roger R. Gomis, Don X. Nguyen, Andy J. Minn, Marc Joan van de Vijver, William Levi Gerald, John A. Foekens, Joan Massague;
Genes that mediate breast cancer metastasis to the brain.
Nature 459:1005-1009(2009)


Louis Guillou, Philippe Terrier, Alain Aurias;
Strong smooth muscle differentiation is dependent on myocardin gene amplification in most human retroperitoneal leiomyosarcomas.
Cancer Res. 69:2269-2278(2009)



switch in melanoma can be overcome by cotargeting MEK and IGF-1R/PI3K.
Cancer Cell 18:683-695(2010)


Julie Adam, Ann Hedley, Gabriela Kalna, Ian Philip Mark Tomlinson, Patrick John Pollard, David George Watson, Ralph J. DeBerardinis, Tomer Shlomi, Eytan Ruppin, Eyal Gottlieb;
Haem oxygenase is synthetically lethal with the tumour suppressor fumarate hydratase.
Nature 477:225-228(2011)


Tetsuya Oguri, Hiroaki Ozasa, Takehiro Uemura, Daishi Kasai, Mikinori Miyazaki, Ken Maeno, Shigeki Sato;
Over-expression of MDR1 in amrubicinol-resistant lung cancer cells.
Cancer Chemother. Pharmacol. 68:669-676(2011)


Pedro G. Ferreira, Matthias Barann, Thomas Wieland, Liliana Greger, Maarten van Iterson, Jonas Carlsson Almlof, Paolo Ribeca, Irina Pulyakhina, Daniela Esser, Thomas Giger, Andrew Tikhonov ...Show all 48 authors... , Marc Sultan, Gabrielle Bertier, Daniel Geoffrey MacArthur, Monkol Lek, Esther Lizano, Henk P.J. Buermans, Ismael Padioleau, Thomas Schwarzmayr, Olof Karlberg, Halit Ongen, Helena Kilpinen, Sergi Beltran, Marta Gut, Katja Kahlem, Vyacheslav Amstislavskiy, Oliver Stegle, Matti Pirinen, Stephen B. Montgomery, Peter Donnelly, Mark Ian McCarthy, Paul Flicek, Tim-Matthias Strom, Hans Lehrach, Stefan Schreiber, Ralf Sudbrak, Angel Carracedo, Stylianos E. Antonarakis, Robert Hasler, Ann-Christine Syvanen, Gert-Jan B. van Ommen, Alvis Brazma, Thomas Meitinger, Philip Rosenstiel, Roderic Guigo, Ivo G. Gut, Xavier Estivill, Emmanouil Theophilos Dermitzakis; Show fewer authors
Geuvadis Consortium
Transcriptome and genome sequencing uncovers functional variation in humans.
Nature 501:506-511(2013)


Christer Thomsen, Soheila Dolatabadi, Emma Jonasson, Chieh-Yuan Li, David Ruff, Shiaw-Min Chen, Pierre Aman;
Normal and functional TP53 in genetically stable myxoid/round cell liposarcoma.
PLoS ONE 9:e113110.1-e113110.7(2014)


Hangnoh Lee, Charles Joel McManus, Dong-Yeon Cho, Matthew Eaton, Fioranna Renda, Maria Patrizia Somma, Lucy Cherbas, Gemma May, Sara Powell, Da-Yu Zhang, Li-Jun Zhan ...Show all 20 authors... , Alissa Resch, Justen Andrews, Susan E. Celniker, Peter Cherbas, Teresa Maria Przytycka, Maurizio Gatti, Brian Oliver, Brenton R. Graveley, David M. MacAlpine; Show fewer authors
DNA copy number evolution in Drosophila cell lines.
Genome Biol. 15:R70.1-R70.20(2014)


Yasuhiro Takashima, Ge Guo, Remco Loos, Jennifer Nichols, Gabriella Ficz, Felix Krueger, David Oxley, Fatima Santos, James Clarke, William Mansfield, Wolf Reik ...Show all 13 authors... , Paul Bertone, Austin Gerard Smith; Show fewer authors
Resetting transcription factor control circuitry toward ground-state pluripotency in human.
Cell 158:1254-1269(2014)


Leora Witkowski, Jian Carrot-Zhang, Steffen Albrecht, Somayyeh Fahiminiya, Nancy Hamel, Eva Tomiak, David Grynspan, Emmanouil Saloustros, Javad Nadaf, Barbara Rivera, Catherine Gilpin ...Show all 42 authors... , Ester Castellsague, Rachel Silva-Smith, Francois Plourde, Mona Wu, Avi Saskin, Madeleine Arseneault, Rouzan G. Karabakhtsian, Elizabeth A. Reilly, Frederick R. Ueland, Anna Margiolaki, Kitty Pavlakis, Sharon M. Castellino, Janez Lamovec, Helen J. Mackay, Lawrence M. Roth, Thomas M. Ulbright, Tracey A. Bender, Vassilis Georgoulias, Michel Longy, Andrew Berchuck, Marc Tischkowitz, Inga Nagel, Reiner Siebert, Colin J.R. Stewart, Jocelyne Arseneau, Wilson Glenn McCluggage, Blaise A. Clarke, Yasser Riazalhosseini, Martin Hasselblatt, Jacek Majewski, William David Foulkes; Show fewer authors
Germline and somatic SMARCA4 mutations characterize small cell carcinoma of the ovary, hypercalcemic type.
Nat. Genet. 46:438-443(2014)


John J. Fak, Eiko Nishiuchi, Constantin Nicolae Takacs, Maria Teresa Catanese, Ype Peter de Jong, Ira M. Jacobson, Charles Moen Rice, Robert Bernard Darnell;
Hepatitis C virus RNA functionally sequesters miR-122.
Cell 160:1099-1110(2015)



literature.
Cancer Res. 77:2784-2788(2017)


Tetsuhiro Kikuchi, Asuka Morizane, Daisuke Doi, Hiroaki Magotani, Hirotaka Onoe, Takuya Hayashi, Hiroshi Mizuma, Sayuki Takara, Ryosuke Takahashi, Haruhisa Inoue, Satoshi Morita ...Show all 16 authors... , Michio Yamamoto, Keisuke Okita, Masato Nakagawa, Malin Parmar, Jun Takahashi; Show fewer authors
Human iPS cell-derived dopaminergic neurons function in a primate Parkinson's disease model.
Nature 548:592-596(2017)




Cancer Cell 33:386-400.e5(2018)



pathogenic THAP11 functions in gene expression and cell proliferation.
PLoS ONE 15:e0224646.1-e0224646.26(2020)

Cross-references
Cell line collections (Providers) IZSLER; BS TCL 103
KCB; KCB 202003YJ
KCLB; 30055
Millipore; SCC438
Cell line databases/resources IGRhCellID; Calu3
LINCS_HMS; 50010
LINCS_LDP; LCL-1631
Lonza; 342
TOKU-E; 768
Chemistry resources PharmacoDB; Calu3_179_2019
PubChem_Cell_line; CVCL_0609
Encyclopedic resources Wikidata; Q52392355
Gene expression databases GEO; GSM159358
GEO; GSM253347
GEO; GSM353233
GEO; GSM434276
GEO; GSM513948
GEO; GSM514318
GEO; GSM784249
GEO; GSM794262
GEO; GSM817064
GEO; GSM827549
GEO; GSM886915
GEO; GSM887981
GEO; GSM1374433
GEO; GSM1374434
GEO; GSM1669658
Metabolomic databases MetaboLights; MTBLS762
MetaboLights; MTBLS2352
MetaboLights; MTBLS2840
Polymorphism and mutation databases IARC_TP53; 21264
LiGeA; CCLE_462
Progenetix; CVCL_0609
Proteomic databases PRIDE; PRD000594
PRIDE; PXD000589
PRIDE; PXD019645
PRIDE; PXD023418
PRIDE; PXD023422
PRIDE; PXD023760
PRIDE; PXD024242
PRIDE; PXD024375
PRIDE; PXD024883
PRIDE; PXD025499
PRIDE; PXD026302
PRIDE; PXD030304
PRIDE; PXD034135
Entry history
Entry creation04-Apr-2012
Last entry update25-Jun-2026
Version number50