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Cellosaurus T47D-KBluc (CVCL_K229)

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Cell line name T47D-KBluc
Synonyms T47D-KBLUC; T47D Kbluc; T47D_KBluc; T47DKBluc; T47DKBLUC
Accession CVCL_K229
Resource Identification Initiative To cite this cell line use: T47D-KBluc (RRID:CVCL_K229)
Comments Part of: JWGray breast cancer cell line panel.
Population: Caucasian.
Characteristics: Used to screen chemicals for estrogenic or anti-estrogenic activity. Selected for responsiveness to 17-beta-estradiol. Transfected with a pGL2.TATA.Inr.luc.neo plasmid which contains three estrogen responsive elements upstream of the luciferase reporter gene (PubMed=15166400).
Doubling time: 70.61 hours (JWGray panel).
Genetic integration: Method=Transfection/transduction; Gene=UniProtKB; P00552; Klebsiella pneumoniae transposon Tn5 neo.
Genetic integration: Method=Transfection/transduction; Gene=UniProtKB; P08659; Photinus pyralis firefly-type luciferase.
Omics: Genomics; Whole exome sequencing.
Omics: Transcriptomics; RNAseq.
Derived from site: Metastatic; Pleural effusion; UBERON=UBERON_0000175.
Sequence variations
  • Mutation; HGNC; HGNC:8975; PIK3CA; Simple; p.His1047Arg (c.3140A>G); ClinVar=VCV000013652; Zygosity=Heterozygous (from parent cell line).
  • Mutation; HGNC; HGNC:11998; TP53; Simple; p.Leu194Phe (c.580C>T); ClinVar=VCV000127817; Zygosity=Homozygous (from parent cell line).
HLA typing Source: PubMed=25960936
Class I
HLA-AA*33:01,33:01
HLA-BB*14:02,14:02
HLA-CC*08:02,08:02
Class II
HLA-DQDQA1*01:02,01:02
DQB1*05:01,05:01
HLA-DRDRB1*01:02,01:02
Disease Invasive breast carcinoma of no special type (NCIt: C4194)
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_0553 (T-47D)
Sex of cell Female
Age at sampling 54Y
Category Cancer cell line
STR profile Source(s): ATCC=CRL-2865

Markers:
AmelogeninX
CSF1PO11,13
D2S133824
D3S135815,17
D5S81812
D7S82011
D8S117913
D13S31712
D16S53910
D18S5117
D19S43314
D21S1128,31
FGA23
Penta D10,12
Penta E7,14
TH016
TPOX11
vWA14

Run an STR similarity search on this cell line
Web pages Info; Synapse; JWGray panel; https://www.synapse.org/#!Synapse:syn2346643/wiki/62255
Publications

PubMed=15166400; DOI=10.1093/toxsci/kfh180
Vickie S. Wilson, Kathy Bobseine, Leon Earl Gray Jr.;
Development and characterization of a cell line that stably expresses an estrogen-responsive luciferase reporter for the detection of estrogen receptor agonist and antagonists.
Toxicol. Sci. 81:69-77(2004)

PubMed=24176112; DOI=10.1186/gb-2013-14-10-r110; PMCID=PMC3937590
Anneleen Daemen, Obi L. Griffith, Laura M. Heiser, Nicholas J. Wang, Oana M. Enache, Zachary Sanborn, Francois Pepin, Steffen Durinck, James E. Korkola, Malachi Griffith, Joe S. Hur ...Show all 27 authors... , Nam Huh, Jongsuk Chung, Leslie Cope, Mary Jo Fackler, Christopher Benedict Umbricht, Saraswati Sukumar, Pankaj Seth, Vikas P. Sukhatme, Lakshmi R. Jakkula, Yi-Ling Lu, Gordon B. Mills, Raymond J. Cho, Eric A. Collisson, Laura J. van 't Veer, Paul T. Spellman, Joe W. Gray; Show fewer authors
Modeling precision treatment of breast cancer.
Genome Biol. 14:R110.1-R110.14(2013)

PubMed=25960936; DOI=10.4161/21624011.2014.954893; PMCID=PMC4355981
Sebastian Boegel, Martin Lower, Thomas Bukur, Ugur Sahin, John C. Castle;
A catalog of HLA type, HLA expression, and neo-epitope candidates in human cancer cell lines.
OncoImmunology 3:e954893.1-e954893.12(2014)

Cross-references
Cell line collections (Providers) ATCC; CRL-2865
Cell line databases/resources CLO; CLO_0037191
cancercelllines; CVCL_K229
LINCS_HMS; 51091
LINCS_LDP; LCL-2064
Encyclopedic resources Wikidata; Q54971561
Gene expression databases GEO; GSM1172905
GEO; GSM1173001
Entry history
Entry creation11-Feb-2013
Last entry update14-Aug-2025
Version number27