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Cellosaurus MCF7EL (CVCL_JX63)

[Text version]
Cell line name MCF7EL
Accession CVCL_JX63
Resource Identification Initiative To cite this cell line use: MCF7EL (RRID:CVCL_JX63)
Comments Part of: MD Anderson Cell Lines Project.
Population: Caucasian.
Characteristics: Upon doxycycline-induction expresses CCNE1. Transfected with a construct containing pTet(tight)-CCNE1-hygR (PubMed=21695458).
Characteristics: Transfected with reverse tetracycline-controlled transactivator (rtTA), a fusion between a mutated version of E.coli TetR and the activating domain of HSV-1 VP16 (from parent cell line).
Genetic integration: Method=Transfection; Gene=HGNC; HGNC:1589; CCNE1.
Genetic integration: Method=Transfection; Gene=UniProtKB; P00557; Escherichia coli hygromycin-B 4-O-kinase (hph) (HygR).
Genetic integration: Method=Transfection; Gene=UniProtKB; P04483; Escherichia coli Tn10 tetR.
Genetic integration: Method=Transfection; Gene=UniProtKB; P06492; Human herpesvirus 1 (HHV-1) VP16 (UL28) (Note=With p.Met1_Arg360del).
Omics: Proteomics; Expression; Reverse-phase protein array.
Derived from site: Metastatic; Pleural effusion; UBERON=UBERON_0000175.
Sequence variations
  • Gene deletion; HGNC; HGNC:1787; CDKN2A; Zygosity=Homozygous (from parent cell line).
  • Mutation; HGNC; HGNC:4172; GATA3; Simple; p.Asp336Glyfs*17 (c.1006dupG); Zygosity=Heterozygous (from parent cell line).
  • Mutation; HGNC; HGNC:8975; PIK3CA; Simple; p.Glu545Lys (c.1633G>A); ClinVar=VCV000013655; Zygosity=Heterozygous (from parent cell line).
Disease Invasive breast carcinoma of no special type (NCIt: C4194)
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_V332 (MCF7 Tet-On)
Sex of cell Female
Age at sampling 69Y
Category Cancer cell line
Web pages Info; MCLP; -; https://tcpaportal.org/mclp/
Publications

PubMed=21695458; DOI=10.1007/s10549-011-1638-4; PMCID=PMC3855283
Angela Nanos-Webb, Natalie A. Jabbour, Asha S. Multani, Hannah Wingate, Nassima Oumata, Herve Galons, Benoit Joseph, Laurent Meijer, Kelly K. Hunt, Khandan Keyomarsi;
Targeting low molecular weight cyclin E (LMW-E) in breast cancer.
Breast Cancer Res. Treat. 132:575-588(2012)

PubMed=28196595; DOI=10.1016/j.ccell.2017.01.005; PMCID=PMC5501076
Jun Li, Wei Zhao, Rehan Akbani, Wen-Bin Liu, Zhen-Lin Ju, Shi-Yun Ling, Christopher P. Vellano, Paul Roebuck, Qing-Hua Yu, A. Karina Eterovic, Lauren Averett Byers ...Show all 25 authors... , Michael A. Davies, Wan-Leng Deng, Y.N. Vashisht Gopal, Guo Chen, Erika Maria von Euw, Dennis Joseph Slamon, Dylan Conklin, John Victor Heymach, Adi F. Gazdar, John D. Minna, Jeffrey N. Myers, Yi-Ling Lu, Gordon B. Mills, Han Liang; Show fewer authors
Characterization of human cancer cell lines by reverse-phase protein arrays.
Cancer Cell 31:225-239(2017)

Cross-references
Cell line databases/resources cancercelllines; CVCL_JX63
Encyclopedic resources Wikidata; Q54904474
Entry history
Entry creation22-Aug-2017
Last entry update14-Aug-2025
Version number16