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Cellosaurus WTC-mEGFP-ACTB-cl184 (CVCL_JM16)

[Text version]
Cell line name WTC-mEGFP-ACTB-cl184
Synonyms ACTB-GFP WTC; AICS-0016-184; AICS0016; AICS-0016; ACTB1 cl. 184; AICSi002-A-3; UCSFi001-A-3
Accession CVCL_JM16
Resource Identification Initiative To cite this cell line use: WTC-mEGFP-ACTB-cl184 (RRID:CVCL_JM16)
Comments Part of: Allen Cell Collection.
Population: Japanese.
Characteristics: Using CRISPR/Cas9 ACTB has been endogenously tagged in one allele at the N-terminus with mEGFP.
Genetic integration: Method=CRISPR/Cas9; Gene=FPbase; QKFJN; mEGFP (Note=Enhanced GFP).
Omics: Genomics; Whole exome sequencing.
Derived from site: In situ; Leg, skin; UBERON=UBERON_0001511.
Cell type: Fibroblast of skin; CL=CL_0002620.
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_Y803 (GM25256)
Sex of cell Male
Age at sampling 30Y
Category Induced pluripotent stem cell
Web pages Info; Allen Institute; -; https://www.allencell.org/cell-catalog.html
Info; Allen Institute; -; https://www.allencell.org/genomics.html
Publications

PubMed=28814507; DOI=10.1091/mbc.E17-03-0209; PMCID=PMC5638588
Brock Roberts, Amanda Haupt, Andrew Tucker, Tanya Grancharova, Joy Arakaki, Margaret A. Fuqua, Angelique M. Nelson, Caroline Hookway, Susan A. Ludmann, Irina A. Mueller, Rui-An Yang ...Show all 14 authors... , Rick Horwitz, Susanne Marie Rafelski, Ruwanthi N. Gunawardane; Show fewer authors
Systematic gene tagging using CRISPR/Cas9 in human stem cells to illuminate cell organization.
Mol. Biol. Cell 28:2854-2874(2017)

PubMed=34019794; DOI=10.1016/j.cell.2021.04.034
Pablo Hofbauer, Stefan M. Jahnel, Nora Papai, Magdalena Giesshammer, Alison Deyett, Clara Schmidt, Mirjam Penc, Katherina Tavernini, Nastasja Grdseloff, Christy Meledeth, Lavinia Ceci Ginistrelli ...Show all 15 authors... , Claudia Ctortecka, Sejla Salic, Maria Novatchkova, Sasha Mendjan; Show fewer authors
Cardioids reveal self-organizing principles of human cardiogenesis.
Cell 184:3299-3317.e22(2021)

Cross-references
Cell line collections (Providers) Coriell; AICS-0016
Cell line databases/resources hPSCreg; UCSFi001-A-3
Biological sample resources BioSamples; SAMEA6298548
Encyclopedic resources Wikidata; Q54994540
Entry history
Entry creation15-May-2017
Last entry update10-Apr-2025
Version number15