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Cellosaurus BFF_NCC2 (CVCL_JJ85)

[Text version]
Cell line name BFF_NCC2
Synonyms Bovine Fetal Fibroblasts_NCC2
Accession CVCL_JJ85
Resource Identification Initiative To cite this cell line use: BFF_NCC2 (RRID:CVCL_JJ85)
Comments Genetic integration: Method=Transduction; Gene=HGNC; HGNC:1582; CCND1.
Genetic integration: Method=Transduction; Gene=HGNC; HGNC:1773; CDK4 (Note=With p.Arg24Cys).
Genetic integration: Method=Transduction; Gene=HGNC; HGNC:11730; TERT.
Genetic integration: Method=Transduction; Gene=UniProtKB; P33967; Bacillus cereus Bsr.
Genetic integration: Method=Transduction; Gene=UniProtKB; P00557; Escherichia coli hygromycin-B 4-O-kinase (hph) (HygR).
Genetic integration: Method=Transduction; Gene=UniProtKB; P13249; Streptomyces alboniger pac (PuroR).
Derived from site: In situ; Fetus; UBERON=UBERON_0000323.
Cell type: Fibroblast; CL=CL_0000057.
Species of origin Bos taurus (Bovine) (NCBI Taxonomy: 9913)
Originate from same individual CVCL_JH58 ! BFF_NCC1
Sex of cell Female
Age at sampling Fetus
Category Telomerase immortalized cell line
Publications

PubMed=24589663; DOI=10.1016/j.jbiotec.2014.02.017
Kenichiro Donai, Tohru Kiyono, Takahiro Eitsuka, Yi-Jie Guo, Kengo Kuroda, Hideko Sone, Emiko Isogai, Tomokazu Fukuda;
Bovine and porcine fibroblasts can be immortalized with intact karyotype by the expression of mutant cyclin dependent kinase 4, cyclin D, and telomerase.
J. Biotechnol. 176:50-57(2014)

Cross-references
Encyclopedic resources Wikidata; Q54796187
Entry history
Entry creation15-May-2017
Last entry update14-Aug-2025
Version number10