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Cellosaurus Hep-2018 (CVCL_JG89)

[Text version]
Cell line name Hep-2018
Synonyms Hep2018; HepaMN
Accession CVCL_JG89
Resource Identification Initiative To cite this cell line use: Hep-2018 (RRID:CVCL_JG89)
Comments Characteristics: The CCDN1, CDK4 and TERT genes expression can be silenced by administration of tetracycline/doxycycline through a Tet-Off system (PubMed=33060611).
Genetic integration: Method=Transduction; Gene=HGNC; HGNC:1582; CCND1.
Genetic integration: Method=Transduction; Gene=HGNC; HGNC:1773; CDK4 (Note=With p.Arg24Cys).
Genetic integration: Method=Transduction; Gene=HGNC; HGNC:11730; TERT.
Genetic integration: Method=Transduction; Gene=UniProtKB; P04483; Escherichia coli Tn10 tetR.
Genetic integration: Method=Transduction; Gene=UniProtKB; P06492; Human herpesvirus 1 VP16 (UL28) (Note=With p.Met1_Arg360del).
Omics: Transcriptomics; Microarray.
Derived from site: In situ; Liver; UBERON=UBERON_0002107.
Cell type: Hepatocyte; CL=CL_0000182.
Disease Biliary atresia (NCIt: C34421)
Isolated biliary atresia (ORDO: Orphanet_30391)
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Sex of cell Female
Age at sampling 4Y
Category Telomerase immortalized cell line
STR profile Source(s): JCRB=JCRB1697

Markers:
AmelogeninX
CSF1PO10,12
D5S81811,13
D7S82010,11
D13S3179,11
D16S5399
TH016,9
TPOX8
vWA17,19

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Publications

PubMed=33060611; DOI=10.1038/s41598-020-73992-3; PMCID=PMC7567112
Nishiwaki M., Toyoda M., Oishi Y., Ishida S., Horiuchi S.-i., Makino-Itou H., Kimura T., Ohno S.-i., Ohkura T., Enosawa S., Akutsu H., Nakazawa A., Kasahara M., Kiyono T., Umezawa A.
Immortalization of human hepatocytes from biliary atresia with CDK4(R24C), cyclin D1, and TERT for cytochrome P450 induction testing.
Sci. Rep. 10:17503.1-17503.13(2020)

Cross-references
Cell line collections (Providers) JCRB; JCRB1697
Encyclopedic resources Wikidata; Q54882758
Entry history
Entry creation15-May-2017
Last entry update10-Apr-2025
Version number13