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Cellosaurus Hep-2018 (CVCL_JG89)

[Text version]
Cell line name Hep-2018
Synonyms Hep2018; HepaMN
Accession CVCL_JG89
Resource Identification Initiative To cite this cell line use: Hep-2018 (RRID:CVCL_JG89)
Comments Characteristics: The CCDN1, CDK4 and TERT genes expression can be silenced by administration of tetracycline/doxycycline through a Tet-Off system (PubMed=33060611).
Genetic integration: Method=Transduction; Gene=HGNC; HGNC:1582; CCND1.
Genetic integration: Method=Transduction; Gene=HGNC; HGNC:1773; CDK4 (Note=With p.Arg24Cys).
Genetic integration: Method=Transduction; Gene=HGNC; HGNC:11730; TERT.
Genetic integration: Method=Transduction; Gene=UniProtKB; P04483; Escherichia coli Tn10 tetR.
Genetic integration: Method=Transduction; Gene=UniProtKB; P06492; Human herpesvirus 1 (HHV-1) VP16 (UL28) (Note=With p.Met1_Arg360del).
Omics: Transcriptomics; Microarray.
Derived from site: In situ; Liver; UBERON=UBERON_0002107.
Cell type: Hepatocyte; CL=CL_0000182.
Disease Biliary atresia (NCIt: C34421)
Isolated biliary atresia (ORDO: Orphanet_30391)
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Sex of cell Female
Age at sampling 4Y
Category Telomerase immortalized cell line
STR profile Source(s): JCRB=JCRB1697

Markers:
AmelogeninX
CSF1PO10,12
D5S81811,13
D7S82010,11
D13S3179,11
D16S5399
TH016,9
TPOX8
vWA17,19

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Publications

PubMed=33060611; DOI=10.1038/s41598-020-73992-3; PMCID=PMC7567112
Manami Nishiwaki, Masashi Toyoda, Yoshie Oishi, Seiichi Ishida, Shin-ichiro Horiuchi, Hatsune Makino-Itou, Tohru Kimura, Shin-ichi Ohno, Takashi Ohkura, Shin Enosawa, Hidenori Akutsu ...Show all 15 authors... , Atsuko Nakazawa, Mureo Kasahara, Tohru Kiyono, Akihiro Umezawa; Show fewer authors
Immortalization of human hepatocytes from biliary atresia with CDK4(R24C), cyclin D1, and TERT for cytochrome P450 induction testing.
Sci. Rep. 10:17503.1-17503.13(2020)

Cross-references
Cell line collections (Providers) JCRB; JCRB1697
Encyclopedic resources Wikidata; Q54882758
Entry history
Entry creation15-May-2017
Last entry update14-Aug-2025
Version number14