Cellosaurus logo
expasy logo

Cellosaurus Ras[V12]-wts[RNAi] (CVCL_IY71)

[Text version]
Cell line name Ras[V12]-wts[RNAi]
Synonyms Ras[v12];wts[RNAi]; WRR-1
Accession CVCL_IY71
Resource Identification Initiative To cite this cell line use: Ras[V12]-wts[RNAi] (RRID:CVCL_IY71)
Comments Group: Insect cell line.
Characteristics: Established from embryos carrying Act5C-GAL4, UAS-Ras[V12] and UAS-wts[RNAi] transposons.
Doubling time: 2 days (DGRC=189).
Genetic integration: Method=Transfection/transduction; Gene=FlyBase_Gene; FBgn0003205; Ras85D (Note=With p.Gly12Val).
Genetic integration: Method=Transfection/transduction; Gene=FPbase; 1XF1B; GFP (Note=avGFP; original A.victoria green fluorescent protein).
Genetic integration: Method=Transfection/transduction; Gene=UniProtKB; P04386; Yeast GAL4.
Omics: Transcriptomics; RNAseq.
Derived from site: In situ; Whole embryo; UBERON=UBERON_0000922.
Species of origin Drosophila melanogaster (Fruit fly) (NCBI Taxonomy: 7227)
Sex of cell Male
Age at sampling Embryonic stage
Category Transformed cell line
Publications

PubMed=18670627; DOI=10.1371/journal.pgen.1000142; PMCID=PMC2474701
Amanda Alice Simcox, Sayan Mitra, Sharon L. Truesdell, Litty Paul, Ting Chen, Jonathan P. Butchar, Steven E. Justiniano;
Efficient genetic method for establishing Drosophila cell lines unlocks the potential to create lines of specific genotypes.
PLoS Genet. 4:e1000142.1-e1000142.12(2008)

PubMed=26772746; DOI=10.1534/g3.115.023366; PMCID=PMC4777130
Marcus Stoiber, Susan E. Celniker, Lucy Cherbas, Ben Brown, Peter Cherbas;
Diverse hormone response networks in 41 independent Drosophila cell lines.
G3 (Bethesda) 6:683-694(2016)

Cross-references
Cell line collections (Providers) DGRC; 189
Cell line databases/resources FlyBase_Cell_line; FBtc0000189
Encyclopedic resources Wikidata; Q54949226
Entry history
Entry creation15-May-2017
Last entry update10-Apr-2025
Version number11