Cellosaurus EndoC-betaH3 (CVCL_IS72)
| Cell line name | EndoC-betaH3 |
|---|---|
| Synonyms | EndoC-BH3 |
| Accession | CVCL_IS72 |
| Resource Identification Initiative | To cite this cell line use: EndoC-betaH3 (RRID:CVCL_IS72) |
| Comments | Characteristics: The lentiviral vector insert containing the SV40 LT and hTERT can be excised by the Cre recombinase upon addition of tamixofen. Following excision of immortalizing transgenes, cell proliferation sharply decreases and in parallel there is a massive enhancement of beta cell-specific features such as increased INS gene expression. Genetic integration: Method=Transduction; Gene=HGNC; HGNC:11730; TERT. Genetic integration: Method=Transduction; Gene=MGI; MGI:1352467; Esr1 (Note=Ligand binding domain; with p.Gly525Arg. Fused to Cre = CreERT2). Genetic integration: Method=Transduction; Gene=UniProtKB; P06956; Escherichia phage P1 cre (Note=Fused to ligand domain of mouse Esr1 = CreERT2). Genetic integration: Method=Transduction; Gene=UniProtKB; P03070; SV40 large T antigen. Transformant: NCBI_TaxID; 1891767; Simian virus 40 (SV40). Derived from site: In situ; Fetal pancreas, islets of Langerhans; UBERON=UBERON_0000006. Cell type: Pancreatic beta cell; CL=CL_0000169. |
| Species of origin | Homo sapiens (Human) (NCBI Taxonomy: 9606) |
| Hierarchy | Parent: CVCL_IS71 (EndoC-betaH2) |
| Sex of cell | Sex unspecified |
| Age at sampling | 9FW |
| Category | Conditionally immortalized cell line |
| Web pages | Provider; Human Cell Design; -; https://www.humancelldesign.com/endoc-bh3/ |
| Publications | PubMed=26909308; DOI=10.1016/j.molmet.2015.09.008; PMCID=PMC4731729 |
| Cross-references | |
| Encyclopedic resources | Wikidata; Q54832324 |
| Entry history | |
| Entry creation | 03-Mar-2017 |
| Last entry update | 10-Apr-2025 |
| Version number | 12 |