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Cellosaurus HMC3 (CVCL_II76)

[Text version]
Cell line name HMC3
Synonyms Human Microglia Clone 3; CHME-3; CHME3
Accession CVCL_II76
Resource Identification Initiative To cite this cell line use: HMC3 (RRID:CVCL_II76)
Comments Virology: Susceptible to infection by Chikungunya virus. Supports viral replication with no signifcant loss in cell viability until 72 hours post infection (PubMed=35352315).
Genetic integration: Method=Transfection; Gene=UniProtKB; P03070; SV40 large T antigen.
Transformant: NCBI_TaxID; 1891767; Simian virus 40 (SV40).
Derived from site: In situ; Fetal brain; UBERON=UBERON_0006238.
Cell type: Microglial cell; CL=CL_0000129.
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Children:
CVCL_D9WB (Ubigene HMC3 CLEC3B KO)
Sex of cell Sex unspecified
Age at sampling 8-10FW
Category Transformed cell line
STR profile Source(s): ATCC=CRL-3304; CLS=300102

Markers:
AmelogeninX
CSF1PO10,11
D2S133817,25
D3S135816,18
D5S81811,12
D6S104311
D7S8209,11
D8S117913,14
D12S39116,21
D13S31711
D16S53912,13
D18S5118
D19S43315,15.2
D21S1130,31.2
FGA21,25
Penta D10,14
Penta E7,13
TH016
TPOX8,9
vWA17,19

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Publications

PubMed=7478261; DOI=10.1016/0304-3940(94)11792-H
Janabi N., Peudenier S., Heron B., Ng K.H., Tardieu M.
Establishment of human microglial cell lines after transfection of primary cultures of embryonic microglial cells with the SV40 large T antigen.
Neurosci. Lett. 195:105-108(1995)

PubMed=16371321; DOI=10.1007/BF03033980
Lindberg C., Hjorth E., Post C., Winblad B., Schultzberg M.
Cytokine production by a human microglial cell line: effects of beta-amyloid and alpha-melanocyte-stimulating hormone.
Neurotox. Res. 8:267-276(2005)

PubMed=20375612; DOI=10.1159/000235563
Li B., Bedard K., Sorce S., Hinz B., Dubois-Dauphin M., Krause K.-H.
NOX4 expression in human microglia leads to constitutive generation of reactive oxygen species and to constitutive IL-6 expression.
J. Innate Immun. 1:570-581(2009)

PubMed=30200996; DOI=10.1186/s12974-018-1288-0; PMCID=PMC6131758
Dello Russo C., Cappoli N., Coletta I., Mezzogori D., Paciello F., Pozzoli G., Navarra P., Battaglia A.
The human microglial HMC3 cell line: where do we stand? A systematic literature review.
J. Neuroinflammation 15:259.1-259.24(2018)

PubMed=35352315; DOI=10.1007/s13365-022-01070-7
Qadri S.W., Kumar N., Santhoshkumar R., Desai A., Ravi V., Venkataswamy M.M.
Infection of human microglial cell line CHME-3 to study neuropathogenesis of Chikungunya virus.
J. Neurovirol. 28:374-382(2022)

Cross-references
Cell line collections (Providers) ATCC; CRL-3304
CLS; 300102
Ubigene; YC-C131
Encyclopedic resources Wikidata; Q54889911
Entry history
Entry creation01-Dec-2016
Last entry update10-Apr-2025
Version number13