| Cell line name |
HMC3 |
| Synonyms |
Human Microglia Clone 3; CHME-3; CHME3 |
| Accession |
CVCL_II76 |
| Resource Identification Initiative |
To cite this cell line use: HMC3 (RRID:CVCL_II76) |
| Comments |
Virology: Susceptible to infection by Chikungunya virus. Supports viral replication with no signifcant loss in cell viability until 72 hours post infection (PubMed=35352315). Genetic integration: Method=Transfection; Gene=UniProtKB; P03070; Simian virus 40 (SV40) large T antigen. Transformant: NCBI_TaxID; 1891767; Simian virus 40 (SV40). Derived from site: In situ; Fetal brain; UBERON=UBERON_0006238. Cell type: Microglial cell; CL=CL_0000129. |
| Species of origin |
Homo sapiens (Human)
(NCBI Taxonomy: 9606) |
| Hierarchy |
Children:
|
| Sex of cell |
Sex unspecified |
| Age at sampling |
8-10FW |
| Category |
Transformed cell line |
| STR profile |
Source(s): ATCC=CRL-3304; CLS=300102
Markers:| Amelogenin | X |
| CSF1PO | 10,11 |
| D2S1338 | 17,25 |
| D3S1358 | 16,18 |
| D5S818 | 11,12 |
| D6S1043 | 11 |
| D7S820 | 9,11 |
| D8S1179 | 13,14 |
| D12S391 | 16,21 |
| D13S317 | 11 |
| D16S539 | 12,13 |
| D18S51 | 18 |
| D19S433 | 15,15.2 |
| D21S11 | 30,31.2 |
| FGA | 21,25 |
| Penta D | 10,14 |
| Penta E | 7,13 |
| TH01 | 6 |
| TPOX | 8,9 |
| vWA | 17,19 |
Run an STR similarity search on this cell line |
| Publications | PubMed=7478261; DOI=10.1016/0304-3940(94)11792-H Nazila Janabi, Sylviane Peudenier, Benedicte Heron, Kim Heng Ng, Marc Tardieu; Establishment of human microglial cell lines after transfection of primary cultures of embryonic microglial cells with the SV40 large T antigen. Neurosci. Lett. 195:105-108(1995) PubMed=16371321; DOI=10.1007/BF03033980 Catharina Lindberg, Erik Hjorth, Claes Post, Bengt Winblad, Marianne Schultzberg; Cytokine production by a human microglial cell line: effects of beta-amyloid and alpha-melanocyte-stimulating hormone. Neurotox. Res. 8:267-276(2005) PubMed=20375612; DOI=10.1159/000235563 Bin Li, Karen Bedard, Silvia Sorce, Boris Hinz, Michel Dubois-Dauphin, Karl-Heinz Krause; NOX4 expression in human microglia leads to constitutive generation of reactive oxygen species and to constitutive IL-6 expression. J. Innate Immun. 1:570-581(2009) PubMed=30200996; DOI=10.1186/s12974-018-1288-0; PMCID=PMC6131758 Cinzia Dello Russo, Natalia Cappoli, Isabella Coletta, Daniele Mezzogori, Fabiola Paciello, Giacomo Pozzoli, Pierluigi Navarra, Alessandra Battaglia; The human microglial HMC3 cell line: where do we stand? A systematic literature review. J. Neuroinflammation 15:259.1-259.24(2018) PubMed=35352315; DOI=10.1007/s13365-022-01070-7 Syed Wasifa Qadri, Narendra Kumar, Rashmi Santhoshkumar, Anita Desai, Vasanthapuram Ravi, Manjunatha M. Venkataswamy; Infection of human microglial cell line CHME-3 to study neuropathogenesis of Chikungunya virus. J. Neurovirol. 28:374-382(2022) |
| Cross-references |
| Cell line collections (Providers) |
ATCC; CRL-3304
CLS; 300102
Ubigene; YC-C131
|
| Encyclopedic resources |
Wikidata; Q54889911
|
| Entry history |
| Entry creation | 01-Dec-2016 |
| Last entry update | 14-Aug-2025 |
| Version number | 14 |
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