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Cellosaurus HCT 116 EP300(-) rescued (CVCL_HG30)

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Cell line name HCT 116 EP300(-) rescued
Synonyms HCT 116 p300 KO Rescue
Accession CVCL_HG30
Resource Identification Initiative To cite this cell line use: HCT 116 EP300(-) rescued (RRID:CVCL_HG30)
Comments Population: Caucasian.
Characteristics: HCT 116 is hemizygous for EP300. This cell line is KO for the single allele and is rescued by transfection of a wild-type copy of EP300 (PubMed=15123817).
Knockout cell: Method=Homologous recombination; HGNC; HGNC:3373; EP300.
Genetic integration: Method=Transfection; Gene=HGNC; HGNC:3373; EP300.
Genetic integration: Method=Transfection; Gene=UniProtKB; P00552; Klebsiella pneumoniae transposon Tn5 neo.
Derived from site: In situ; Colon; UBERON=UBERON_0001155.
Sequence variations
  • Mutation; HGNC; HGNC:173; ACVR2A; Simple; p.Lys437Argfs*5 (c.1310delA); dbSNP=rs764719749; Zygosity=Homozygous (from parent cell line).
  • Mutation; HGNC; HGNC:1101; BRCA2; Simple; p.Ile2675Aspfs*6 (c.8021dupA) (c.8021_8022insA); ClinVar=VCV000267050; Zygosity=Heterozygous (from parent cell line).
  • Mutation; HGNC; HGNC:1787; CDKN2A; Simple; p.Arg24Serfs*20 (c.68dupG) (c.68_69insG) (p.G23fs); Zygosity=Heterozygous (from parent cell line).
  • Mutation; HGNC; HGNC:2514; CTNNB1; Simple; p.Ser45del (c.133_135delTCT); ClinVar=VCV000017576; Zygosity=Heterozygous (from parent cell line).
  • Mutation; HGNC; HGNC:6407; KRAS; Simple; p.Gly13Asp (c.38G>A); ClinVar=VCV000012580; Zygosity=Heterozygous (from parent cell line).
  • Mutation; HGNC; HGNC:8975; PIK3CA; Simple; p.His1047Arg (c.3140A>G); ClinVar=VCV000013652; Zygosity=Heterozygous (from parent cell line).
  • Mutation; HGNC; HGNC:11773; TGFBR2; Simple; p.Lys128Serfs*35 (c.383delA); ClinVar=VCV000477546; Zygosity=Homozygous (from parent cell line).
Disease Colon carcinoma (NCIt: C4910)
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_HG29 (HCT 116 EP300(-) clone F5)
Sex of cell Male
Age at sampling 48Y
Category Cancer cell line
STR profile Source(s): ECACC=17011211

Markers:
AmelogeninX
CSF1PO7,10
D3S135812,17
D5S81810,11
D7S82011,12
D8S117911,14
D13S31710,13
D16S53911,13
D18S5116,17
D21S1129,30
FGA18,23
Penta D9,13
Penta E13,14
TH018,9
TPOX8
vWA18,22

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Publications

PubMed=15123817; DOI=10.1073/pnas.0401002101; PMCID=PMC409928
N. Gopalakrishna Iyer, Suet-Feung Chin, Hilal Ozdag, Yataro Daigo, De-En Hu, Massimiliano Cariati, Kevin Michael Brindle, Samuel Aparicio, Carlos Caldas;
p300 regulates p53-dependent apoptosis after DNA damage in colorectal cancer cells by modulation of PUMA/p21 levels.
Proc. Natl. Acad. Sci. U.S.A. 101:7386-7391(2004)

Cross-references
Cell line collections (Providers) CancerTools; 153186
ECACC; 17011211 - Discontinued
Ximbio; 153186
Cell line databases/resources cancercelllines; CVCL_HG30
Encyclopedic resources Wikidata; Q54881937
Entry history
Entry creation01-Dec-2016
Last entry update14-Aug-2025
Version number19