Cell line name |
HCT 116 EP300(-) clone D10 |
Synonyms |
HCT 116 p300 KO Clone D10; HCT 116 p300 KO [D10]; p300 KO Clone D10 |
Accession |
CVCL_HG27 |
Resource Identification Initiative |
To cite this cell line use: HCT 116 EP300(-) clone D10 (RRID:CVCL_HG27) |
Comments |
Population: Caucasian. Characteristics: HCT 116 is hemizygous for EP300. This cell line is KO for the single allele. Knockout cell: Method=Homologous recombination; HGNC; HGNC:3373; EP300. Derived from site: In situ; Colon; UBERON=UBERON_0001155. |
Sequence variations |
- Mutation; HGNC; HGNC:173; ACVR2A; Simple; p.Lys437Argfs*5 (c.1310delA); dbSNP=rs764719749; Zygosity=Homozygous (from parent cell line).
- Mutation; HGNC; HGNC:1101; BRCA2; Simple; p.Ile2675Aspfs*6 (c.8021dupA) (c.8021_8022insA); ClinVar=VCV000267050; Zygosity=Heterozygous (from parent cell line).
- Mutation; HGNC; HGNC:1787; CDKN2A; Simple; p.Arg24Serfs*20 (c.68dupG) (c.68_69insG) (p.G23fs); Zygosity=Heterozygous (from parent cell line).
- Mutation; HGNC; HGNC:2514; CTNNB1; Simple; p.Ser45del (c.133_135delTCT); ClinVar=VCV000017576; Zygosity=Heterozygous (from parent cell line).
- Mutation; HGNC; HGNC:3373; EP300; Simple; p.Met1470Cysfs*22 (c.4408delA); Zygosity=Heterozygous (from parent cell line).
- Mutation; HGNC; HGNC:3373; EP300; Simple; p.Asn1700Thrfs*9 (c.5099delA); Zygosity=Heterozygous (from parent cell line).
- Mutation; HGNC; HGNC:6407; KRAS; Simple; p.Gly13Asp (c.38G>A); ClinVar=VCV000012580; Zygosity=Heterozygous (from parent cell line).
- Mutation; HGNC; HGNC:8975; PIK3CA; Simple; p.His1047Arg (c.3140A>G); ClinVar=VCV000013652; Zygosity=Heterozygous (from parent cell line).
- Mutation; HGNC; HGNC:9277; PPM1D; Simple; p.Leu450Ter (c.1349delT) (p.Leu450fs) (c.1344delT); Zygosity=Heterozygous (from parent cell line).
- Mutation; HGNC; HGNC:11773; TGFBR2; Simple; p.Lys128Serfs*35 (c.383delA); ClinVar=VCV000477546; Zygosity=Homozygous (from parent cell line).
|
Disease |
Colon carcinoma (NCIt: C4910) |
Species of origin |
Homo sapiens (Human)
(NCBI Taxonomy: 9606) |
Hierarchy |
Parent: CVCL_HG26 (HCT 116 EP300 WT) |
Sex of cell |
Male |
Age at sampling |
48Y |
Category |
Cancer cell line |
STR profile |
Source(s): ECACC=17011201
Markers:Amelogenin | X |
CSF1PO | 7,10 |
D3S1358 | 12,17 |
D5S818 | 10,11 |
D7S820 | 11,12 |
D8S1179 | 11,14 |
D13S317 | 10,13 |
D16S539 | 11,13.1 |
D18S51 | 11,14 |
D21S11 | 29,30 |
FGA | 18,23 |
Penta D | 9,13 |
Penta E | 13,14 |
TH01 | 8,9 |
TPOX | 8 |
vWA | 18,21 |
Run an STR similarity search on this cell line |
Publications | PubMed=15123817; DOI=10.1073/pnas.0401002101; PMCID=PMC409928 Iyer N.G., Chin S.-F., Ozdag H., Daigo Y., Hu D.-E., Cariati M., Brindle K.M., Aparicio S., Caldas C. p300 regulates p53-dependent apoptosis after DNA damage in colorectal cancer cells by modulation of PUMA/p21 levels. Proc. Natl. Acad. Sci. U.S.A. 101:7386-7391(2004) |
Cross-references |
Cell line collections (Providers) |
CancerTools; 151479
ECACC; 17011201 - Discontinued
Ximbio; 151479
|
Cell line databases/resources |
cancercelllines; CVCL_HG27
|
Encyclopedic resources |
Wikidata; Q54881934
|
Entry history |
Entry creation | 01-Dec-2016 |
Last entry update | 19-Dec-2024 |
Version number | 18 |
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