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Cellosaurus RKO BRAF(+/-/-) (CVCL_HE15)

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Cell line name RKO BRAF(+/-/-)
Synonyms BRAF (+/-/-) RKO
Accession CVCL_HE15
Resource Identification Initiative To cite this cell line use: RKO BRAF(+/-/-) (RRID:CVCL_HE15)
Comments Characteristics: RKO carries a stable triplication of the BRAF gene with one wild-type (V-600) and two mutant alleles (E-600). This KO cell line carries the wild-type allele.
Knockout cell: Method=Homologous recombination; HGNC; HGNC:1097; BRAF (Note=2 of 3 alleles).
Derived from site: In situ; Colon; UBERON=UBERON_0001155.
Sequence variations
  • Mutation; HGNC; HGNC:173; ACVR2A; Simple; p.Lys437Argfs*5 (c.1310delA); dbSNP=rs764719749; Zygosity=Heterozygous (from parent cell line).
  • Mutation; HGNC; HGNC:8975; PIK3CA; Simple; p.His1047Arg (c.3140A>G); ClinVar=VCV000013652; Zygosity=Heterozygous (from parent cell line).
  • Mutation; HGNC; HGNC:11773; TGFBR2; Simple; p.Lys128Serfs*35 (c.383delA); ClinVar=VCV000477546; Zygosity=Heterozygous (from parent cell line).
  • Mutation; HGNC; HGNC:11773; TGFBR2; Simple; p.Leu452Pro (c.1355T>C); ClinVar=VCV000930524; Zygosity=Heterozygous (from parent cell line).
Disease Colon carcinoma (NCIt: C4910)
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_0504 (RKO)
Sex of cell Sex unspecified
Age at sampling Age unspecified
Category Cancer cell line
Publications

PubMed=19661383; DOI=10.1126/science.1174229; PMCID=PMC2820374
Jiyeon Yun, Carlo Rago, Ian Cheong, Raymond Pagliarini, Philipp Angenendt, Harith Rajagopalan, Kerstin Schmidt, James K.V. Willson, Sanford David Markowitz, Shi-Bin Zhou, Luis Alberto Diaz Jr. ...Show all 16 authors... , Victor E. Velculescu, Christoph Lengauer, Kenneth Wayne Kinzler, Bert Vogelstein, Nickolas Papadopoulos; Show fewer authors
Glucose deprivation contributes to the development of KRAS pathway mutations in tumor cells.
Science 325:1555-1559(2009)

Cross-references
Cell line collections (Providers) Horizon_Discovery; HD+106-003
Cell line databases/resources cancercelllines; CVCL_HE15
Encyclopedic resources Wikidata; Q54950539
Entry history
Entry creation01-Dec-2016
Last entry update19-Dec-2024
Version number13