Cellosaurus cell line HMC-1.2 (CVCL

Cellosaurus HMC-1.2 (CVCL_H205)

[Text version]

Cell line name

HMC-1.2

Synonyms

HMC-1_2; HMC1_2; HMC1.2; HMC-1(560,816); HMC-1-560_816

Accession

CVCL_H205

Resource Identification Initiative

To cite this cell line use: HMC-1.2 (RRID:CVCL_H205)

Comments

Characteristics: Compound 48/80 which is used to promote mast cell degranulation is toxic to this cell line (DOI=10.57844/arcadia-3207-4695).
Omics: Transcriptomics; RNAseq.
Derived from site: In situ; Peripheral blood; UBERON=UBERON_0000178.
Cell type: Mast cell; CL=CL_0000097.

Sequence variations

Mutation; HGNC; HGNC:6342; KIT; Simple; p.Val560Gly (c.1679T>G); ClinVar=VCV000375916; Zygosity=Heterozygous (PubMed=12519307).
Mutation; HGNC; HGNC:6342; KIT; Simple; p.Asp816Val (c.2447A>T); ClinVar=VCV000013852; Zygosity=Heterozygous (PubMed=12519307).

Disease

Mast cell leukemia (NCIt: C3169)
Mast cell leukemia (ORDO: Orphanet_98851)

Species of origin

Homo sapiens (Human) (NCBI Taxonomy: 9606)

Hierarchy

Parent: CVCL_0003 (HMC-1)
Children:

CVCL_E8DV (HMC-1.3 clone 1H9)

CVCL_E8DW (HMC-1.3 clone 2E2)

Sex of cell

Male

Age at sampling

52Y

Category

Cancer cell line

STR profile

Source(s): Millipore=SCC062

Markers:

Amelogenin	X
CSF1PO	10,13
D3S1358	15
D5S818	12,13
D7S820	10,11
D8S1179	12
D13S317	10,11
D16S539	12
D18S51	14,20
D21S11	29
FGA	18,20
Penta D	11
Penta E	9,19
TH01	7,9.3
TPOX	8,11
vWA	17,19

Web pages

Info; protocols.io; -; https://dx.doi.org/10.17504/protocols.io.261ger8xwl47/v1

Publications

PubMed=11861291; DOI=10.1182/blood.V99.5.1741
Yong-Sheng Ma, Shan Zeng, Dean D. Metcalfe, Cem Akin, Sasa Dimitrijevic, Joseph H. Butterfield, Gerald McMahon, B. Jack Longley;
The c-KIT mutation causing human mastocytosis is resistant to STI571 and other KIT kinase inhibitors; kinases with enzymatic site mutations show different inhibitor sensitivity profiles than wild-type kinases and those with regulatory-type mutations.
Blood 99:1741-1744(2002)

PubMed=12519307; DOI=10.1046/j.1365-2567.2003.01559.x; PMCID=PMC1782858
Magnus Sundstrom, Harissios Vliagoftis, Peter Karlberg, Joseph H. Butterfield, Kenneth Nilsson, Dean D. Metcalfe, Gunnar Nilsson;
Functional and phenotypic studies of two variants of a human mast cell line with a distinct set of mutations in the c-kit proto-oncogene.
Immunology 108:89-97(2003)

PubMed=20814942; DOI=10.1002/0471142735.im0737s90; PMCID=PMC2923748
Madeleine Radinger, Bettina Margrethe Jensen, Hye Sun Kuehn, Arnold S. Kirshenbaum, Alasdair M. Gilfillan;
Generation, isolation, and maintenance of human mast cells and mast cell lines derived from peripheral blood or cord blood.
Curr. Protoc. Immunol. 90:7.37.1-7.37.12(2010)

PubMed=25149487; DOI=10.1007/978-1-4939-1173-8_8
Egle Passante;
Mast cell and basophil cell lines: a compendium.
Methods Mol. Biol. 1192:101-113(2014)

PubMed=37025992; DOI=10.3389/fimmu.2023.1078958; PMCID=PMC10071028
Geethani Bandara, Guido H. Falduto, Andrea J. Luker, Yun Bai, Annika Pfeiffer, Justin B. Lack, Dean D. Metcalfe, Ana Olivera;
CRISPR/Cas9-engineering of HMC-1.2 cells renders a human mast cell line with a single D816V-KIT mutation: an improved preclinical model for research on mastocytosis.
Front. Immunol. 14:1078958.1-1078958.17(2023)

DOI=10.57844/arcadia-3207-4695
MaryClare Rollins, Edgard Morazan, Adair L. Borges, Justin Donnelly;
Compound 48/80 is toxic in HMC1.2 and RBL-2H3 cells.
Arcadia 2025:0317.1-0317.18(2025)

Cross-references

Cell line collections (Providers)

Millipore; SCC062

Cell line databases/resources

cancercelllines; CVCL_H205

Encyclopedic resources

Wikidata; Q54889910

Gene expression databases

GEO; GSM6674137
GEO; GSM6674138
GEO; GSM6674139

Polymorphism and mutation databases

Cosmic; 819945
Cosmic; 1476310
Cosmic; 1476422
Cosmic; 1498492

Entry history

Entry creation

11-Feb-2013

Last entry update

10-Apr-2025

Version number