Cellosaurus cell line HMC-1.2 (CVCL

Cellosaurus HMC-1.2 (CVCL_H205)

[Text version]

Cell line name

HMC-1.2

Synonyms

HMC-1_2; HMC1_2; HMC1.2; HMC-1(560,816); HMC-1-560_816

Accession

CVCL_H205

Resource Identification Initiative

To cite this cell line use: HMC-1.2 (RRID:CVCL_H205)

Comments

Characteristics: Compound 48/80 which is used to promote mast cell degranulation is toxic to this cell line (DOI=10.57844/arcadia-3207-4695).
Omics: Transcriptomics; RNAseq.
Derived from site: In situ; Peripheral blood; UBERON=UBERON_0000178.
Cell type: Mast cell; CL=CL_0000097.

Sequence variations

Mutation; HGNC; HGNC:6342; KIT; Simple; p.Val560Gly (c.1679T>G); ClinVar=VCV000375916; Zygosity=Heterozygous (PubMed=12519307).
Mutation; HGNC; HGNC:6342; KIT; Simple; p.Asp816Val (c.2447A>T); ClinVar=VCV000013852; Zygosity=Heterozygous (PubMed=12519307).

Disease

Mast cell leukemia (NCIt: C3169)
Mast cell leukemia (ORDO: Orphanet_98851)

Species of origin

Homo sapiens (Human) (NCBI Taxonomy: 9606)

Hierarchy

Parent: CVCL_0003 (HMC-1)
Children:

CVCL_E8DV (HMC-1.3 clone 1H9)

CVCL_E8DW (HMC-1.3 clone 2E2)

Sex of cell

Male

Age at sampling

52Y

Category

Cancer cell line

STR profile

Source(s): Millipore=SCC062

Markers:

Amelogenin	X
CSF1PO	10,13
D3S1358	15
D5S818	12,13
D7S820	10,11
D8S1179	12
D13S317	10,11
D16S539	12
D18S51	14,20
D21S11	29
FGA	18,20
Penta D	11
Penta E	9,19
TH01	7,9.3
TPOX	8,11
vWA	17,19

Web pages

Info; protocols.io; -; https://dx.doi.org/10.17504/protocols.io.261ger8xwl47/v1

Publications

PubMed=11861291; DOI=10.1182/blood.V99.5.1741
Ma Y.-S., Zeng S., Metcalfe D.D., Akin C., Dimitrijevic S., Butterfield J.H., McMahon G., Longley B.J.
The c-KIT mutation causing human mastocytosis is resistant to STI571 and other KIT kinase inhibitors; kinases with enzymatic site mutations show different inhibitor sensitivity profiles than wild-type kinases and those with regulatory-type mutations.
Blood 99:1741-1744(2002)

PubMed=12519307; DOI=10.1046/j.1365-2567.2003.01559.x; PMCID=PMC1782858
Sundstrom M., Vliagoftis H., Karlberg P., Butterfield J.H., Nilsson K., Metcalfe D.D., Nilsson G.
Functional and phenotypic studies of two variants of a human mast cell line with a distinct set of mutations in the c-kit proto-oncogene.
Immunology 108:89-97(2003)

PubMed=20814942; DOI=10.1002/0471142735.im0737s90; PMCID=PMC2923748
Radinger M., Jensen B.M., Kuehn H.S., Kirshenbaum A.S., Gilfillan A.M.
Generation, isolation, and maintenance of human mast cells and mast cell lines derived from peripheral blood or cord blood.
Curr. Protoc. Immunol. 90:7.37.1-7.37.12(2010)

PubMed=25149487; DOI=10.1007/978-1-4939-1173-8_8
Passante E.
Mast cell and basophil cell lines: a compendium.
Methods Mol. Biol. 1192:101-113(2014)

PubMed=37025992; DOI=10.3389/fimmu.2023.1078958; PMCID=PMC10071028
Bandara G., Falduto G.H., Luker A.J., Bai Y., Pfeiffer A., Lack J.B., Metcalfe D.D., Olivera A.
CRISPR/Cas9-engineering of HMC-1.2 cells renders a human mast cell line with a single D816V-KIT mutation: an improved preclinical model for research on mastocytosis.
Front. Immunol. 14:1078958.1-1078958.17(2023)

DOI=10.57844/arcadia-3207-4695
Rollins M., Morazan E., Borges A.L., Donnelly J.
Compound 48/80 is toxic in HMC1.2 and RBL-2H3 cells.
Arcadia 2025:0317.1-0317.18(2025)

Cross-references

Cell line collections (Providers)

Millipore; SCC062

Cell line databases/resources

cancercelllines; CVCL_H205

Encyclopedic resources

Wikidata; Q54889910

Gene expression databases

GEO; GSM6674137
GEO; GSM6674138
GEO; GSM6674139

Polymorphism and mutation databases

Cosmic; 819945
Cosmic; 1476310
Cosmic; 1476422
Cosmic; 1498492

Entry history

Entry creation

11-Feb-2013

Last entry update

10-Apr-2025

Version number