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Cellosaurus FE-8 (CVCL_GS30)

[Text version]
Cell line name FE-8
Synonyms FE8
Accession CVCL_GS30
Resource Identification Initiative To cite this cell line use: FE-8 (RRID:CVCL_GS30)
Comments Characteristics: Highly tumorigenic in athymic mice and newborn rats (PubMed=3125552).
Selected for resistance to: ChEBI; CHEBI_9555; Tioguanine (6-thioguanine; 6-TG).
Genetic integration: Method=Transfection; Gene=HGNC; HGNC:5173; HRAS (Note=With p.Gly12Val).
Genetic integration: Method=Transfection; Gene=UniProtKB; P00552; Klebsiella pneumoniae transposon Tn5 neo.
Transformant: ChEBI; CHEBI_23994; Ethyl methanesulfonate (Methylsulfonic acid ethyl ester; MSEE).
Derived from site: In situ; Whole embryo; UBERON=UBERON_0000922.
Cell type: Fibroblast; CL=CL_0000057.
Species of origin Rattus norvegicus (Rat) (NCBI Taxonomy: 10116)
Breed/subspecies: Fischer 344; RS=RS_0000215.
Hierarchy Parent: CVCL_4086 (208F)
Children:
CVCL_E9WN (FER1)CVCL_E9WP (FER2)CVCL_E9WR (FER8)
Sex of cell Male
Age at sampling Embryo
Category Transformed cell line
Publications

PubMed=3533793; DOI=10.1002/ijc.2910380513
Sabine Griegel, Otto Traub, Klaus Willecke, Reinhold Schaefer;
Suppression and re-expression of transformed phenotype in hybrids of HA-ras-1-transformed rat-1 cells and early-passage rat embryonic fibroblasts.
Int. J. Cancer 38:697-705(1986)

PubMed=3125552; DOI=10.1073/pnas.85.5.1590; PMCID=PMC279819
Reinhold Schaefer, Jaya Iyer, Elsbeth Iten, Arto C. Nirkko;
Partial reversion of the transformed phenotype in HRAS-transfected tumorigenic cells by transfer of a human gene.
Proc. Natl. Acad. Sci. U.S.A. 85:1590-1594(1988)

Cross-references
Cell line databases/resources TOKU-E; 3546
Encyclopedic resources Wikidata; Q54834714
Entry history
Entry creation26-Sep-2016
Last entry update14-Aug-2025
Version number14