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Cellosaurus TK6261 (CVCL_F6LS)

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Cell line name TK6261
Accession CVCL_F6LS
Resource Identification Initiative To cite this cell line use: TK6261 (RRID:CVCL_F6LS)
Comments Population: Caucasian.
Characteristics: Does not proliferate in 2'-deoxycytidine-hypoxanthine-aminopterin-thymidine medium unless TK1 activity is restored (PubMed=37714828).
Selected for resistance to: ChEBI; CHEBI_9555; Tioguanine (6-thioguanine; 6-TG).
Transformant: NCBI_TaxID; 10376; Epstein-Barr virus (EBV).
Transformant: ChEBI; CHEBI_132982; Acridine half-mustard dihydrochloride (6-chloro-9-[3-(2-chloroethylamino)propylamino]-2-methoxyacridine dihydrochloride; Acridine mutagen ICR 191).
Derived from site: In situ; Spleen; UBERON=UBERON_0002106.
Cell type: B-cell; CL=CL_0000236.
Sequence variations
  • Mutation; HGNC; HGNC:11272; SPTA1; Simple; p.Ser1163Ala (c.3487T>G); ClinVar=VCV000258929; Zygosity=Homozygous (from parent cell line).
  • Mutation; HGNC; HGNC:11830; TK1; Simple; p.Ala78Argfs*27 (c.231insC); Zygosity=Heterozygous (from parent cell line).
  • Mutation; HGNC; HGNC:11830; TK1; Simple_edited; p.Asp104Glyfs*195 (c.311_318del); Zygosity=Heterozygous; Note=By CRISPR/Cas9, introduced on the allele carrying c.640insG (PubMed=37714828).
  • Mutation; HGNC; HGNC:11830; TK1; Simple; p.Glu214Glyfs*153 (c.640insG); Zygosity=Heterozygous (from parent cell line).
Disease Hereditary spherocytosis (NCIt: C97074)
Hereditary spherocytosis (ORDO: Orphanet_822)
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_AR68 (TK6 (IVGT))
Sex of cell Male
Age at sampling 5Y
Category Transformed cell line
Publications

PubMed=37714828; DOI=10.1038/s41467-023-41048-5; PMCID=PMC10504326
Akiko Tomita, Hiroyuki Sasanuma, Tomoo Owa, Yuka Nakazawa, Mayuko Shimada, Takahiro Fukuoka, Tomoo Ogi, Shinichiro Nakada;
Inducing multiple nicks promotes interhomolog homologous recombination to correct heterozygous mutations in somatic cells.
Nat. Commun. 14:5607-5607(2023)

Entry history
Entry creation22-Oct-2026
Last entry update22-Oct-2026
Version number1