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Cellosaurus HeLa ATG101 KO+Halo-LC3 (CVCL_F6LP)

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Cell line name HeLa ATG101 KO+Halo-LC3
Synonyms ATG101-KO+Halo-LC3B
Accession CVCL_F6LP
Resource Identification Initiative To cite this cell line use: HeLa ATG101 KO+Halo-LC3 (RRID:CVCL_F6LP)
Comments Population: African American.
Characteristics: Transduced with a pCDH1-CMV-HT-LC3-SV40-Hygro construct (Addgene #182045).
Characteristics: Stably expresses HaloTag-LC3 to measure autophagic flux.
Characteristics: Polyclonal population.
Knockout cell: Method=CRISPR/Cas9; HGNC; HGNC:25679; ATG101.
Genetic integration: Method=Transduction; Gene=HGNC; HGNC:13352; MAP1LC3B.
Genetic integration: Method=Transduction; Gene=UniProtKB; P00557; Escherichia coli hygromycin-B 4-O-kinase (hph) (HygR).
Genetic integration: Method=Transduction; Gene=UniProtKB; P0A3G2; Rhodococcus rhodochrous dhaA (Note=Mutated = HaloTag).
Genetic integration: Method=Transduction; Gene=UniProtKB; P13249; Streptomyces alboniger pac (PuroR).
Transformant: NCBI_TaxID; 333761; Human papillomavirus type 18 (HPV18).
Miscellaneous: Cell line information from personal communication of Yongjia, Duan.
Derived from site: In situ; Uterus, cervix; UBERON=UBERON_0000002.
Disease Human papillomavirus-related endocervical adenocarcinoma (NCIt: C27677)
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_F6LN (HeLa ATG101 KO)
Children:
CVCL_F6LR (HeLa ATG101 KO+Halo-LC3+HA-ATG101-W110D|F112D)CVCL_F6LQ (HeLa ATG101 KO+Halo-LC3+HA-ATG101-WT)
Sex of cell Female
Age at sampling 30Y6M
Category Cancer cell line
Entry history
Entry creation22-Oct-2026
Last entry update22-Oct-2026
Version number1