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Cellosaurus PTEN-/-KRAS(G12V)-Luc (CVCL_F6KW)

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Cell line name PTEN-/-KRAS(G12V)-Luc
Accession CVCL_F6KW
Resource Identification Initiative To cite this cell line use: PTEN-/-KRAS(G12V)-Luc (RRID:CVCL_F6KW)
Comments Characteristics: Tumorigenic in nude-Foxn1nu mice (PubMed=26497685).
Knockout cell: Method=Homologous recombination; HGNC; HGNC:9588; PTEN.
Genetic integration: Method=Transduction; Gene=HGNC; HGNC:6407; KRAS (Note=With p.Gly12Val).
Genetic integration: Method=Transduction; Gene=UniProtKB; P00557; Escherichia coli hygromycin-B 4-O-kinase (hph) (HygR).
Genetic integration: Method=Transduction; Gene=UniProtKB; P00552; Klebsiella pneumoniae transposon Tn5 neo.
Genetic integration: Method=Transduction; Gene=UniProtKB; P08659; Photinus pyralis firefly-type luciferase.
Derived from site: In situ; Breast, epithelium; UBERON=UBERON_0008367.
Sequence variations
  • Gene deletion; HGNC; HGNC:1787; CDKN2A; Zygosity=Homozygous (from parent cell line).
  • Gene deletion; HGNC; HGNC:1788; CDKN2B; Zygosity=Homozygous (from parent cell line).
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_F6KS (MCF10A PTEN-/- KRas(G12V) clone 1)
Sex of cell Female
Age at sampling 36Y
Category Spontaneously immortalized cell line
Publications

PubMed=26497685; DOI=10.18632/oncotarget.6159; PMCID=PMC4742101
Keyata N. Thompson, Rebecca A. Whipple, Jennifer R. Yoon, Michael Lipsky, Monica S. Charpentier, Amanda E. Boggs, Kristi R. Chakrabarti, Lekhana Bhandary, Lindsay K. Hessler, Stuart S. Martin, Michele I. Vitolo;
The combinatorial activation of the PI3K and Ras/MAPK pathways is sufficient for aggressive tumor formation, while individual pathway activation supports cell persistence.
Oncotarget 6:35231-35246(2015)

Entry history
Entry creation22-Oct-2026
Last entry update22-Oct-2026
Version number1