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Cellosaurus HEK293TEG (CVCL_F6KJ)

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Cell line name HEK293TEG
Accession CVCL_F6KJ
Resource Identification Initiative To cite this cell line use: HEK293TEG (RRID:CVCL_F6KJ)
Comments Characteristics: Using CRISPR/Cas9 a mNeonGreen-P2A-3xFLAG construct was introduced at the N-terminus of EEA1 (DOI=10.1101/2024.04.19.590338).
Characteristics: Using CRISPR/Cas9 a 3xHA-P2A-mNeonGreen construct was introduced at the C-terminus of TMEM115 (DOI=10.1101/2024.04.19.590338).
Genetic integration: Method=CRISPR/Cas9; Gene=FPbase; ZRKRV; mNeonGreen (Note=Derivative of Branchiostoma lanceolatum LanYFP).
Genetic integration: Method=Transfection; Gene=UniProtKB; P00552; Klebsiella pneumoniae transposon Tn5 neo.
Genetic integration: Method=Transfection; Gene=UniProtKB; P03070; Simian virus 40 (SV40) large T antigen.
Transformant: NCBI_TaxID; 28285; Human adenovirus C serotype 5.
Transformant: NCBI_TaxID; 1891767; Simian virus 40 (SV40) (Note=tsA).
Miscellaneous: Cell line information from personal communication of Tao, Fu.
Derived from site: In situ; Fetal kidney; UBERON=UBERON_0002113.
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_0063 (HEK293T)
Children:
CVCL_F6KK (HEK293TEG DNAJC13 KO)
Sex of cell Female
Age at sampling Fetus
Category Transformed cell line
Publications

PubMed=38659763; DOI=10.1101/2024.04.19.590338; PMCID=PMC11042255
L.Robert Hollingsworth, Priya Veeraraghavan, Joao A. Paulo, J.Wade Harper;
Spatiotemporal proteomic profiling of cellular responses to NLRP3 agonists.
bioRxiv no_volume:2024.04.19.590338-2024.04.19.590338(2024)

Entry history
Entry creation22-Oct-2026
Last entry update22-Oct-2026
Version number1