Cellosaurus cell line HyCyte A-549 stable cell line for hSMO overexpression (CVCL

Cross-references
Cell line name	HyCyte A-549 stable cell line for hSMO overexpression
Synonyms	A-549 stable cell line for hSMO overexpression
Accession	CVCL_F4VN
Resource Identification Initiative	To cite this cell line use: HyCyte A-549 stable cell line for hSMO overexpression (RRID:CVCL_F4VN)
Comments	Population: Caucasian. Characteristics: Transduced with pLV[Exp]-CMV>SMO[NM_005631.5]-CBH>EGFP-2A-Puro. Doubling time: 36-48 hours (Hysigen=CGSC-M25821). Genetic integration: Method=Transduction; Gene=FPbase; R9NL8; EGFP. Genetic integration: Method=Transduction; Gene=HGNC; HGNC:11119; SMO. Genetic integration: Method=Transduction; Gene=UniProtKB; P13249; Streptomyces alboniger pac (PuroR). Derived from site: In situ; Lung; UBERON=UBERON_0002048.
Sequence variations	Mutation; HGNC; HGNC:6407; KRAS; Simple; p.Gly12Ser (c.34G>A); ClinVar=VCV000012584; Zygosity=Homozygous (from parent cell line). Mutation; HGNC; HGNC:11389; STK11; Simple; p.Gln37Ter (c.109C>T); ClinVar=VCV000376334; Zygosity=Homozygous (from parent cell line). Mutation; HGNC; HGNC:11998; TP53; None_reported; -; Zygosity=- (from parent cell line).
Disease	Lung adenocarcinoma (NCIt: C3512)
Species of origin	Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy	Parent: CVCL_0023 (A-549)
Sex of cell	Male
Age at sampling	58Y
Category	Cancer cell line
Cell line collections (Providers)	Hysigen; CGSC-M25821
Entry history
Entry creation	25-Jun-2026
Last entry update	25-Jun-2026
Version number	1