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Cellosaurus HyCyte MDCK.2 stable cell line for hMDR1 overexpression (CVCL_F4VL)

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Cell line name HyCyte MDCK.2 stable cell line for hMDR1 overexpression
Synonyms MDCK.2 stable cell line for hMDR1 overexpression
Accession CVCL_F4VL
Resource Identification Initiative To cite this cell line use: HyCyte MDCK.2 stable cell line for hMDR1 overexpression (RRID:CVCL_F4VL)
Comments Characteristics: Transfected with pPB[Exp]-EF1A>EGFP-CAG>MDR1[NM_001348946.2]-PGK>Puro.
Doubling time: 24-36 hours (Hysigen=CGSC-M25771).
Genetic integration: Method=PiggyBac transposition; Gene=FPbase; R9NL8; EGFP.
Genetic integration: Method=PiggyBac transposition; Gene=HGNC; HGNC:40; ABCB1.
Genetic integration: Method=PiggyBac transposition; Gene=UniProtKB; P13249; Streptomyces alboniger pac (PuroR).
Derived from site: In situ; Kidney; UBERON=UBERON_0002113.
Cell type: Epithelial cell of kidney; CL=CL_0002518.
Species of origin Canis lupus familiaris (Dog) (Canis familiaris) (NCBI Taxonomy: 9615)
Breed/subspecies: Cocker Spaniel; VBO=VBO_0200372.
Hierarchy Parent: CVCL_B034 (MDCK.2)
Sex of cell Female
Age at sampling Adult
Category Spontaneously immortalized cell line
Cross-references
Cell line collections (Providers) Hysigen; CGSC-M25771
Entry history
Entry creation25-Jun-2026
Last entry update25-Jun-2026
Version number1