Cellosaurus cell line HyCyte A-549 stable cell line for hFFAR4 overexpression (CVCL

Cross-references
Cell line name	HyCyte A-549 stable cell line for hFFAR4 overexpression
Synonyms	A-549 stable cell line for hFFAR4 overexpression
Accession	CVCL_F4SA
Resource Identification Initiative	To cite this cell line use: HyCyte A-549 stable cell line for hFFAR4 overexpression (RRID:CVCL_F4SA)
Comments	Population: Caucasian. Characteristics: Transfected with pPB[Exp]-EF1A>EGFP-CAG>hFFAR4[NM_181745.4]-PGK>Puro. Doubling time: 36-48 hours (Hysigen=CGSC-M33451). Genetic integration: Method=PiggyBac transposition; Gene=FPbase; R9NL8; EGFP. Genetic integration: Method=PiggyBac transposition; Gene=HGNC; HGNC:19061; FFAR4. Genetic integration: Method=PiggyBac transposition; Gene=UniProtKB; P13249; Streptomyces alboniger pac (PuroR). Derived from site: In situ; Lung; UBERON=UBERON_0002048.
Sequence variations	Mutation; HGNC; HGNC:6407; KRAS; Simple; p.Gly12Ser (c.34G>A); ClinVar=VCV000012584; Zygosity=Homozygous (from parent cell line). Mutation; HGNC; HGNC:11389; STK11; Simple; p.Gln37Ter (c.109C>T); ClinVar=VCV000376334; Zygosity=Homozygous (from parent cell line). Mutation; HGNC; HGNC:11998; TP53; None_reported; -; Zygosity=- (from parent cell line).
Disease	Lung adenocarcinoma (NCIt: C3512)
Species of origin	Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy	Parent: CVCL_0023 (A-549)
Sex of cell	Male
Age at sampling	58Y
Category	Cancer cell line
Cell line collections (Providers)	Hysigen; CGSC-M33451
Entry history
Entry creation	25-Jun-2026
Last entry update	25-Jun-2026
Version number	1