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Cellosaurus HyCyte U-251MG stable cell line for hTLR2 overexpression (CVCL_F4PX)

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Cell line name HyCyte U-251MG stable cell line for hTLR2 overexpression
Synonyms U-251MG stable cell line for hTLR2 overexpression
Accession CVCL_F4PX
Resource Identification Initiative To cite this cell line use: HyCyte U-251MG stable cell line for hTLR2 overexpression (RRID:CVCL_F4PX)
Comments Population: Caucasian.
Characteristics: Transfected with pPB[Exp]-EF1A>EGFP-CAG>hTLR2[NM_001318796.2]-PGK>Puro.
Doubling time: 24-36 hours (Hysigen=CGSC-M30351).
Genetic integration: Method=PiggyBac transposition; Gene=FPbase; R9NL8; EGFP.
Genetic integration: Method=PiggyBac transposition; Gene=HGNC; HGNC:11848; TLR2.
Genetic integration: Method=PiggyBac transposition; Gene=UniProtKB; P13249; Streptomyces alboniger pac (PuroR).
Derived from site: In situ; Brain, parietal lobe; UBERON=UBERON_0001872.
Sequence variations
  • Mutation; HGNC; HGNC:5382; IDH1; None_reported; -; Zygosity=- (from parent cell line).
  • Mutation; HGNC; HGNC:9588; PTEN; Simple; p.Glu242Valfs*15 (c.723_724dupTG); ClinVar=VCV000536553; Zygosity=Unspecified (from parent cell line).
  • Mutation; HGNC; HGNC:11730; TERT; Simple; c.1-124C>T (c.228C>T) (C228T); Zygosity=Unspecified; Note=In promoter (from parent cell line).
  • Mutation; HGNC; HGNC:11998; TP53; Simple; p.Arg273His (c.818G>A); ClinVar=VCV000012366; Zygosity=Homozygous (from parent cell line).
Disease Astrocytoma (NCIt: C60781)
Astrocytoma (ORDO: Orphanet_94)
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_0021 (U-251MG)
Sex of cell Male
Age at sampling 75Y
Category Cancer cell line
Cross-references
Cell line collections (Providers) Hysigen; CGSC-M30351
Entry history
Entry creation25-Jun-2026
Last entry update25-Jun-2026
Version number1