Cellosaurus cell line HyCyte MDA-MB-231 KO-hCPB1 (CVCL

Cross-references
Cell line name	HyCyte MDA-MB-231 KO-hCPB1
Synonyms	hCPB1 knockout MDA-MB-231 cell line
Accession	CVCL_F4F2
Resource Identification Initiative	To cite this cell line use: HyCyte MDA-MB-231 KO-hCPB1 (RRID:CVCL_F4F2)
Comments	Population: Caucasian. Doubling time: 24-36 hours (Hysigen=CGKO-M3345). Knockout cell: Method=CRISPR/Cas9; HGNC; HGNC:2299; CPB1. Derived from site: Metastatic; Pleural effusion; UBERON=UBERON_0000175.
Sequence variations	Gene deletion; HGNC; HGNC:1787; CDKN2A; Zygosity=Homozygous (from parent cell line). Gene deletion; HGNC; HGNC:1788; CDKN2B; Zygosity=Homozygous (from parent cell line). Mutation; HGNC; HGNC:1097; BRAF; Simple; p.Gly464Val (c.1391G>T); ClinVar=VCV000040364; Zygosity=Heterozygous (from parent cell line). Mutation; HGNC; HGNC:6407; KRAS; Simple; p.Gly13Asp (c.38G>A); ClinVar=VCV000012580; Zygosity=Heterozygous (from parent cell line). Mutation; HGNC; HGNC:11730; TERT; Simple; c.1-124C>T (c.228C>T) (C228T); Zygosity=Unspecified; Note=In promoter (from parent cell line). Mutation; HGNC; HGNC:11998; TP53; Simple; p.Arg280Lys (c.839G>A); ClinVar=VCV000376657; Zygosity=Homozygous (from parent cell line).
Disease	Breast adenocarcinoma (NCIt: C5214)
Species of origin	Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy	Parent: CVCL_0062 (MDA-MB-231)
Sex of cell	Female
Age at sampling	51Y
Category	Cancer cell line
Cell line collections (Providers)	Hysigen; CGKO-M3345
Entry history
Entry creation	25-Jun-2026
Last entry update	25-Jun-2026
Version number	1