Cellosaurus C6/36-HE8-Act::Cas9-2A-Neo (CVCL_F1ZQ)
| Cell line name | C6/36-HE8-Act::Cas9-2A-Neo |
|---|---|
| Synonyms | C6-36-HE8-Act::Cas9-2A-Neo |
| Accession | CVCL_F1ZQ |
| Resource Identification Initiative | To cite this cell line use: C6/36-HE8-Act::Cas9-2A-Neo (RRID:CVCL_F1ZQ) |
| Comments | Group: Insect cell line. Characteristics: Modified using a Minos mediated integration cassette (MiMIC) to deliver a recombination-mediated cassette (RMCE) acceptor, to add a docking site for recombination via the bacteriophage FC31 integrase. Characteristics: A N-3xFLAG-spCas9-T2A-Neo construct was integrated in the Act5C locus (PubMed=34819517). Genetic integration: Method=Transfection; Gene=FPbase; ZERB6; mCherry (Note=Red fluorescent protein derived from drFP583 (dsRed)). Genetic integration: Method=Transfection; Gene=UniProtKB; P00552; Klebsiella pneumoniae transposon Tn5 neo. Genetic integration: Method=Transfection; Gene=UniProtKB; Q99ZW2; Streptomyces pyogenes Cas9. |
| Species of origin | Aedes albopictus (Asian tiger mosquito) (Stegomyia albopicta) (NCBI Taxonomy: 7160) |
| Hierarchy | Parent: CVCL_B3N5 (C6/36-HE8) |
| Sex of cell | Sex unspecified |
| Age at sampling | Neonate larva |
| Category | Spontaneously immortalized cell line |
| Publications | PubMed=34819517; DOI=10.1038/s41467-021-27129-3; PMCID=PMC8613219 |
| Cross-references | |
| Cell line collections (Providers) | DGRC; 363 |
| Cell line databases/resources | FlyBase_Cell_line; FBtc0000363 |
| Entry history | |
| Entry creation | 31-Mar-2026 |
| Last entry update | 31-Mar-2026 |
| Version number | 1 |