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Cellosaurus HeLa S3 ATG13 KO+Halo-LC3+HA-ATG13-HF:DD (CVCL_F1Z1)

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Cell line name HeLa S3 ATG13 KO+Halo-LC3+HA-ATG13-HF:DD
Accession CVCL_F1Z1
Resource Identification Initiative To cite this cell line use: HeLa S3 ATG13 KO+Halo-LC3+HA-ATG13-HF:DD (RRID:CVCL_F1Z1)
Comments Population: African American.
Characteristics: Stably expresses HaloTag-LC3 to measure autophagic flux.
Knockout cell: Method=CRISPR/Cas9; HGNC; HGNC:29091; ATG13.
Genetic integration: Method=Transduction; Gene=HGNC; HGNC:29091; ATG13 (Note=With p.His214Asp and p.Phe215Asp and a N-terminal HA tag).
Genetic integration: Method=Transduction; Gene=RGD; 621315; Map1lc3b.
Genetic integration: Method=Transduction; Gene=UniProtKB; P00557; Escherichia coli hygromycin-B 4-O-kinase (hph) (HygR).
Genetic integration: Method=Transduction; Gene=UniProtKB; P0A3G2; Rhodococcus rhodochrous dhaA (Note=Mutated = HaloTag).
Transformant: NCBI_TaxID; 333761; Human papillomavirus type 18 (HPV18).
Derived from site: In situ; Uterus, cervix; UBERON=UBERON_0000002.
Disease Human papillomavirus-related endocervical adenocarcinoma (NCIt: C27677)
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_F1YZ (HeLa S3 ATG13 KO+HaloTag-LC3)
Sex of cell Female
Age at sampling 30Y6M
Category Cancer cell line
Publications

DOI=10.1101/2025.11.07.687251
Yong-Jia Duan, Ye Lu, Sanjoy Paul, Johannes Betz, Lea P. Wilhelm, Annan Samuel Isaac Cook, Xue-Feng Ren, Elias Adriaenssens, Sascha Martens, Ian G. Ganley, Gerhard Hummer, James Herschel Hurley;
Reconstitution of multistep recruitment of ULK1 to membranes in autophagy.
bioRxiv 2025:11.07.687251-11.07.687251(2025)

Entry history
Entry creation27-Nov-2025
Last entry update27-Nov-2025
Version number1