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Cellosaurus N86/8 (CVCL_E9T5)

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Cell line name N86/8
Accession CVCL_E9T5
Resource Identification Initiative To cite this cell line use: N86/8 (RRID:CVCL_E9T5)
Comments Part of: UC Davis/NIH NeuroMab Facility hybridoma collection.
Monoclonal antibody isotype: IgG2a.
Monoclonal antibody target: FPbase; 1XF1B; GFP (Note=avGFP; original A.victoria green fluorescent protein. Also reacts with CFP and YFP).
Species of origin Mus musculus (Mouse) (NCBI Taxonomy: 10090)
Hierarchy Parent: CVCL_2199 (Sp2/0-Ag14)
Category Hybridoma
Web pages Info; NeuroMab; -; https://neuromab.ucdavis.edu/datasheet/N86_8.pdf
Publications

PubMed=30667360; DOI=10.7554/eLife.43322; PMCID=PMC6377228
Nicolas P. Andrews, Justin Xavier Boeckman, Colleen F. Manning, Joe T. Nguyen, Hannah Bechtold, Camelia Dumitras, Belvin Gong, Kimberly Nguyen, Deborah van der List, Karl D. Murray, JoAnne Engebrecht, James S. Trimmer;
A toolbox of IgG subclass-switched recombinant monoclonal antibodies for enhanced multiplex immunolabeling of brain.
eLife 8:e43322.1-e43322.27(2019)

PubMed=37758930; DOI=10.1038/s41598-023-43233-4; PMCID=PMC10533561
Keith Gerald Mitchell, Belvin Gong, Samuel S. Hunter, Diana Burkart-Waco, Clara Elizabeth Gavira-O'Neill, Kayla M. Templeton, Madeline E. Goethel, Malgorzata Bzymek, Leah M. MacNiven, Karl D. Murray, Matthew L. Settles ...Show all 13 authors... , Lutz Froenicke, James S. Trimmer; Show fewer authors
High-volume hybridoma sequencing on the NeuroMabSeq platform enables efficient generation of recombinant monoclonal antibodies and scFvs for neuroscience research.
Sci. Rep. 13:16200.1-16200.20(2023)

Cross-references
Cell line collections (Providers) DSHB; N86-8
MMRRC; MMRRC:066054-UCD
Biological sample resources ABCD; ABCD_AV444
Entry history
Entry creation14-Aug-2025
Last entry update14-Aug-2025
Version number1