Cellosaurus 4434-EKAREV-NLS (CVCL_E9CL)
| Cell line name | 4434-EKAREV-NLS |
|---|---|
| Accession | CVCL_E9CL |
| Resource Identification Initiative | To cite this cell line use: 4434-EKAREV-NLS (RRID:CVCL_E9CL) |
| Comments | Characteristics: Carries the FRET biosensor EKARrEV-NLS, which consists of a donor fluorescent protein CFP, an ERK substrate peptide derived from Cdc25, an optimized linker, a FHA1 phosphate binding domain, an acceptor fluorescent protein YFP and a nuclear localization signal (PubMed=25873177). Genetic integration: Method=PiggyBac transposition; Gene=FPbase; ZRLU4; CFP (Note=Cyan fluorescent protein derived from GFP). Genetic integration: Method=PiggyBac transposition; Gene=FPbase; 8DNLG; YFP (Note=EYFP, yellow fluorescent protein derived from GFP). Genetic integration: Method=Transgenic mouse; Gene=MGI; MGI:1352467; Esr1 (Note=Ligand binding domain; with p.Gly525Arg. Fused to Cre = CreERT2). Breed/subspecies: C57BL/6 BRAF+/LSL-BRAFV600E;Tyr::CreERT2+/o transgenic. |
| Sequence variations | |
| Disease | Mouse melanoma (NCIt: C21790) |
| Species of origin | Mus musculus (Mouse) (NCBI Taxonomy: 10090) |
| Hierarchy | Parent: CVCL_E9CI (4434) |
| Sex of cell | Sex unspecified |
| Age at sampling | Age unspecified |
| Category | Cancer cell line |
| Publications | PubMed=25873177; DOI=10.1016/j.ccell.2015.03.008; PMCID=PMC4402404 |
| Entry history | |
| Entry creation | 14-Aug-2025 |
| Last entry update | 14-Aug-2025 |
| Version number | 1 |